Background:

(±)14(15)-EET-d₁₁is intended for use as an internal standard for the quantification of 14(15)-EET by GC- or LC-MS. 14(S),15(R)-EET and 14(R),15(S)-EET are formedviaepoxidation of arachidonic acid by a variety of cytochrome P450 (CYP) isoforms.¹14(S),15(R)-EET is produced at a higher proportion by CYP2C23, whereas 14(R),15(S)-EET is produced at a greater proportion by CYP2B2 and CYP2C24.
(±)14(15)-EET-d₁₁MaxSpec^?standard is a quantitative grade standard of (±)14(15)-EET-d₁₁that has been prepared specifically for mass spectrometry and related applications where quantitative reproducibility is required. The solution has been prepared gravimetrically and is supplied in a deactivated glass ampule sealed under argon. The concentration was verified by comparison to an independently prepared calibration standard. This (±)14(15)-EET-d₁₁MaxSpec^?standard is guaranteed to meet identity, purity, stability, and concentration specifications and is provided with a batch-specific certificate of analysis. Ongoing stability testing is performed to ensure the concentration remains accurate throughout the shelf life of the product.Note: The amount of solution added to the vial is in excess of the listed amount. Therefore, it is necessary to accurately measure volumes for preparation of calibration standards. Follow recommended storage and handling conditions to maintain product quality.

1.Capdevila, J.H., Falck, J.R., and Harris, R.C.Cytochrome P450 and arachidonic acid bioactivation: Molecular and functional properties of the arachidonate monooxygenaseJ. Lipid Res.41(2)163-181(2000)