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Cy5,5 NHS ester(non-sulfonated)
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Cy5,5 NHS ester(non-sulfonated)图片
规格:98%
分子量:716.31
包装与价格:
包装价格(元)
5mg电议
25mg电议

产品介绍
Cy5.5-NHS ester?is a near-infrared amine reactive dye for labeling amino groups of peptides, proteins and oligonucleotides.
货号:ajcx11092
CAS:1469277-96-0
分子式:C44H46ClN3O4
分子量:716.31
溶解度:≥ 35.8mg/mL in DMSO
纯度:98%
存储:Store at -20°C
库存:现货

Background:

Cy5.5-NHS ester is a near-infrared amine reactive dye for labeling amino groups of peptides, proteins and oligonucleotides. Cy5.5-NHS ester is suitable for fluorescence analysis when the background fluorescence interferes. It can also be used for in vivo imaging experiments. Cy5.5-NHS ester can be dissolved in DMF and DMSO, the maximum excitation light and maximum emission light are around 675 nm and 693 nm, respectively.[1]

In vitro and in vivo experiment indicated that Cy5.5-NHS ester is a nice near-infrared fluorescent dye, which could be used for tumor targeting as well as examining the effect of D-glucose or L-glucose on 2-NBDG accumulation. Moreover, Cy5.5-NHS ester reacts swiftly that the tumor/muscle contrast could be clearly visualized from 30 min up to 24 h pi of 0.5 nmol Cy5.5-NHS. The tumor uptake of Cy5.5-NHS reached a maximum 30 minutes pi.[2]

参考文献:
[1].Ju HR, et al. Non-invasive optical imaging of cathepsin B with activatable fluorogenic nanoprobes in various metastatic models. Biomaterials. 2014. 35: 2302-2311.
[2].Zhen C. et al. Near-Infrared Fluorescent Deoxyglucose Analog for Tumor Optical Imaging in Cell Culture and in Living Mice. Bioconjug Chem. 2006 ; 17(3): 662–669.

Protocol:

Cell experiment [1]:

Cell lines

U87MG

Preparation Method

U87MG cells (1 × 105) were cultured for 24 hours, then the cells were washed with PBS and then incubated at 37 °C in the presence of 100 nM Cy5.5-NHS ester for 10 minutes. After the incubation period, cells were washed three times with ice- cold PBS.

Reaction Conditions

The fluorescence signal of the cells was recorded using an Axiovert 200M fluorescence microscope equipped with a Cy5.5 filter set.

Applications

Cy5.5-NHS ester could be used for fluorescence microscopy imaging study with tumor cell lines such as U87MG, C6, A375M, B16F10 and MDAMB-435, as well as for examining the effect of D-glucose or L-glucose on 2-NBDG accumulation.

Animal experiment [1]:

Animal models

Female athymic nude mice (nu/nu), 4–6 weeks

Preparation Method

Mice were subcutaneously injected with 5 × 106U87MG glioblastoma cells suspended in 100 μL of PBS. When the tumors reached 0.4–0.6 cm in diameter, the tumor bearing mice were then injected via tail vein with Cy5.5-NHS ester and subjected to optical imaging at various time points postinjection (pi).

Dosage form

0.5 nmol

Applications

Cy5.5-NHS ester could be took advantage of tumor targeting, and the tumor/muscle contrast was clearly visible as early as 30 min postinjection. The highest U87MG tumor/muscle ratio of 3.34 ± 0.23 were achieved 24 hours post-injection for Cy5.5-NHS.

参考文献:

[1]. Zhen C. et al. Near-Infrared Fluorescent Deoxyglucose Analog for Tumor Optical Imaging in Cell Culture and in Living Mice. Bioconjug Chem. 2006 ; 17(3): 662–669.