A chromogenic alkaline phosphatase substrate
货号:ajcx25294
CAS:333338-18-4
分子式:C6H4NO6P·2Na [6H2O]
分子量：371.1
溶解度:Ethanol: 10 mg/ml,PBS (pH 7.2): 10 mg/ml
纯度：98%
存储：Store at -20°C
库存：现货

Background:

4-Nitrophenyl phosphate (pNPP) is a chromogenic substrate for alkaline phosphatase.¹Upon hydrolysis by alkaline phosphatase, 4-nitrophenol is released, which can be quantified by colorimetric detection at 405 nm as a measure of alkaline phosphatase activity. It has been commonly used in ELISAs.

1.Lorenz, U.Protein tyrosine phosphatase assaysCurr. Protoc. Immunol.9311.17.11-11.17.12(2011)