BRD0539 是一种可渗透细胞的，无毒的 CRISPR-Cas9 抑制剂。在体外 DNA 裂解试验中，BRD0539 可抑制化脓性链球菌 Cas9 (SpCas9)，IC50 为 22 μM。
货号:ajcx36986
CAS:1403838-79-8
分子式:C25H25FN2O3S
分子量：452.54
溶解度:N/A
纯度：98%
存储：Store at -20°C
库存：现货

Background:

BRD0539 is a cell-permeable and non-toxic inhibitor of CRISPR-Cas9. BRD0539 inhibits Streptococcus pyogenes Cas9 (SpCas9) (apparent IC50=22 μM) in an in vitro DNA cleavage assay[1].

BRD0539 dose-dependently blocks the formation of the DNA-bound state in a dose-dependent fashion. BRD0539 impairs the perturbation induced by the 4PAM DNA[1].BRD0539 does not interfere with the SpCas9:gRNA interaction[1].BRD0539 is able to inhibit SpCas9 in the eGFP-disruption assay, BRD0539 is unable to inhibit FnCpf1, a structurally different CRISPR-associated nuclease, in the same assay, further highlighting the specificity of BRD0539[1].

[1]. Basudeb Maji, et al. A High-Throughput Platform to Identify Small-Molecule Inhibitors of CRISPR-Cas9. Cell. 2019 May 2;177(4):1067-1079.e19.