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Enteropeptidase Fluorogenic Substrate(trifluoroacetate salt)
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Enteropeptidase Fluorogenic Substrate(trifluoroacetate salt)图片
规格:98%
分子量:859.7
包装与价格:
包装价格(元)
500ug电议
1mg电议
5mg电议

产品介绍
A fluorogenic substrate of enteropeptidase
货号:ajcx24974
CAS:N/A
分子式:C34H40F3N7O16·XCF3COOH
分子量:859.7
溶解度:DMF: 30 mg/mL,DMSO: 30 mg/mL,Ethanol: 10 mg/mL,PBS (pH 7.2): 2.5 mg/mL
纯度:98%
存储:Store at -20°C
库存:现货

Background:

Enteropeptidase fluorogenic substrate is a substrate for enteropeptidase that contains a 7-amino-4-trifluoromethylcoumarin (AFC) moiety. Enteropeptidase is a serine protease expressed in the proximal small intestine of higher animals that converts inactive trypsinogen to active trypsin by endoproteolytic cleavage.1,2Enteropeptidase recognizes the highly specific amino acid sequence DDDDK on the fluorogenic substrate and cleaves after the lysine residue, releasing the AFC moiety. Enteropeptidase activity is quantified by fluorescent detection of AFC, which displays excitation/emission spectra of 380/500 nm.3


1.Pepeliaev, S., Krahulec, J., ?ernÝ, Z., et al.High level expression of human enteropeptidase light chain in Pichia pastorisJ. Biotechnol.156(1)67-75(2011) 2.Choi, M.-G., Lee, S., Chung, H.-S., et al.A fluorogenic method for measuring enteropeptidase activity: Spectral shift in the emission of GD4K-conjugated 7-amino-4-methylcoumarinBMB Rep.44(7)458-461(2011) 3.Gossrau, R.Cytochemistry of membrane proteasesHistochem J.17(7)737-771(1985)