The mammalian MAP7 family members include MAP7 (ensconsin, E-MAP-115), MAP7D1, MAP7D2, and MAP7D3. All family members share a highly conserved N-terminal coiled-coil motif, which interacts with microtubules and a conserved C-terminal MAP7 coiled-coil domain that is presumed to bind to kinesin-1. MAP7 and MAP7D1 are broadly expressed and have been described to play a role in neuronal developmental processes, including in axonal development in cultured neurons. MAP7D2 on the other hand is predominantly expressed in brain tissues. MAP7D2 interacts with all three kinesin-1 family members and accumulates in the proximal axon through its N-terminal microtubule-binding domain. Depletion of MAP7D2 results in reduced axonal cargo entry and defects in axon formation and outgrowth during early stages of neuronal development. MAP7D2 is a local kinesin-1 regulator that promotes cargo entry into the axon.