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Nefiracetam
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Nefiracetam图片
CAS NO:77191-36-7
包装:50mg, 100mg
包装与价格:
包装价格(元)
Free Sample (0.5-1 mg)电议
10mM*1mL in DMSO电议
50mg电议
100mg电议

产品名称
DZL 221
DM9384
产品介绍

生物活性

Nefiracetam is cognitive enhancer with an IC50 of approximately 150–200 μM for Ro 5-4864. It displays various pharmacological effects. It activates L/N-type calcium channels, cholinergic, monoaminergic and GABAergic systems. It displays potent neuroprotective action in the retinal ischemia-reperfusion model in vivo.


化学数据

分子量246.3
分子式C14H18N2O2
CAS号77191-36-7
纯度>98%
溶解性(25°C)DMSO 25 mg/mL
储存和运输条件固体粉末: -20°C 冷藏长期储存
常温运输及临时存放

实验操作 来自于公开的文献,仅供相同实验参考(如实验材料、目的不同,请参考其他文献)

细胞实验
细胞系Oocytes
方法Transferring the injected oocytes to the recording chamber 24 to 48 hours after incubation and continuously superfused at room temperature (20 to 22 °C) in a standard frog Ringer's solution (115 mM NaCl, 2 mM KCl, 1.8 mM CaCl2, and 5 mM HEPES, pH 7.0). Ca2+ -free extracellular solution consisted of 115 mM NaCl, 2 mM KCl, 5 mM MgCl2, 5 mM HEPES, and 1 mM EGTA, pH 7.0. To remove the effect of the muscarinic ACh receptor, adding 1 μM atropine the extracellular solution. Using two-electrode, voltage-clamp techniques to record ACh-activated currents. Analyzing the currents on a microcomputer using pClamp software. ACh is bath-applied to oocytes. Nefiracetam is dissolved in distilled water at 1 mM for stock solution and diluted into concentrations which are required with the extracellular solution.
浓度~1 μM
处理时间24 hours - 48 hours

动物实验
动物模型Adult male EL mice weighing 25–30 g and adult male DDY mice
配制
剂量
给药处理Administered via i.v. or p.o.

不同实验动物依据体表面积的等效剂量转换表(数据来源于FDA指南)

小鼠大鼠豚鼠仓鼠
重量 (kg)0.020.151.80.40.0810
体表面积 (m2)0.0070.0250.150.050.020.5
Km系数36128520
动物 A (mg/kg) = 动物 B (mg/kg) × 动物 B的Km系数
动物 A的Km系数

例如,依据体表面积折算法,将化合物用于小鼠的剂量20 mg/kg 换算成大鼠的剂量,需要将20 mg/kg 乘以小鼠的Km系数(3),再除以大鼠的Km系数(6),得到化合物用于大鼠的等效剂量为10 mg/kg。


储备液配制

以下数据基于产品分子量,对于特殊产品,请参照COA中的储备液配制条件和说明进行操作。

Concentration / Solvent Volume / Mass1 mg5 mg10 mg
1 mM4.0601 mL20.3004 mL40.6009 mL
5 mM0.812 mL4.0601 mL8.1202 mL
10 mM0.406 mL2.03 mL4.0601 mL