Calcifediol monohydrate (also known as 25-hydroxyvitamin D3; 25-hydroxy VD3) is the major circulating metabolite of vitamin D3 in the blood and is the form that is tested in medicine to determine vitamin D deficiency, namely, the test of 25-hydroxy vitamin D (calcifediol) levels to evaluate how much vitamin D is in the body. It is also an intermediate in the biosynthetic pathway leading to the production of 1,25-dihydroxy vitamin D3, the active form of vitamin D. Calcifediol acts as a competitive inhibitor with an apparent Ki of 3.9 μM. It also suppresses PTH secretion and mRNA (ED50=2 nM). Calcifediol induced CYP24A1 expression with EC50 at 70 nM. Calcifediol stimulated the expression of thrombomodulin with EC50 at 10-100 nM. Confocal microscopy revealed that calcifediol at 0.1 - 10 μM induced VDR translocation into the nucleus dose-dependently; the VDR localization pattern was similar in cells treated with calcitriol.

纯度：≥98%

CAS：63283-36-3