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(S)-4'-nitro-Blebbistatin
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
(S)-4'-nitro-Blebbistatin图片
CAS NO:1621326-32-6
包装与价格:
包装价格(元)
1mg电议
5mg电议
10mg电议

产品介绍
(S)-4'-nitro-Blebbistatin 是一种非细胞毒性、光稳定、荧光和特异性肌球蛋白 II 抑制剂,用于研究肌球蛋白 II 在生理、发育和细胞生物学研究中的特定作用。
Cas No.1621326-32-6
Canonical SMILESO=C1[C@@]2(O)C(N(C3=CC=C([N+]([O-])=O)C=C3)CC2)=NC4=C1C=C(C)C=C4
分子式C18H15N3O4
分子量337.3
溶解度DMF: 20 mg/ml,DMF:PBS(pH 7.2)(1:1): 0.5 mg/ml,DMSO: 12.5 mg/ml
储存条件Store at -20°C
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping ConditionEvaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
产品描述

(S)-4'-nitro-Blebbistatin is a more stable and less phototoxic form of (-)-blebbistatin , which is a selective cell-permeable inhibitor of non-muscle myosin II ATPases.1,2(-)-Blebbistatin rapidly and reversibly inhibits Mg-ATPase activity andin vitromotility of non-muscle myosin IIA and IIB for several species (IC50s = 0.5-5 µM), while poorly inhibiting smooth muscle myosin (IC50= 80 µM).3Through these effects, it blocks apoptosis-related bleb formation, directed cell migration, and cytokinesis in vertebrate cells. However, prolonged exposure to blue light (450-490 nm) results in degradation of blebbistatin to an inactive productviacytotoxic intermediates, which may be problematic for its use in fluorescent live cell imaging applications.4,5The addition of a 4'-nitro group decreases the inherent fluorescence, stabilizes the molecule to circumvent its degradation by prolonged blue light exposure, and decreases its phototoxicity while retaining thein vitroandin vivoactivity of (-)-blebbistatin.6,7(S)-4'-nitro-Blebbistatin has the same stereochemistry as the active (-)-blebbistatin enantiomer.

1.Straight, A.F., Cheung, A., Limouze, J., et al.Dissecting temporal and spatial control of cytokinesis with a myosin II inhibitorScience299(5613)1743-1747(2003) 2.KovÁcs, M., TÓth, J., HetÉnyi, C., et al.Mechanism of blebbistatin inhibition of myosin IIJ. Biol. Chem.279(34)35557-35563(2004) 3.Limouze, J., Straight, A.F., Mitchison, T., et al.Specificity of blebbistatin, an inhibitor of myosin IIJ. Muscle Res. Cell Motil.25(4-5)337-341(2004) 4.Kolega, J.Phototoxicity and photoinactivation of blebbistatin in UV and visible lightBiochem. Biophys. Res. Commun.320(3)1020-1025(2004) 5.Sakamoto, T., Limouze, J., Combs, C.A., et al.Blebbistatin, a myosin II inhibitor, is photoinactivated by blue lightBiochemistry44(2)584-588(2005) 6.KÉpirÓ, M., VÁrkuti, B.H., VÉgner, L., et al.para-Nitroblebbistatin, the non-cytotoxic and photostable myosin II inhibitorAngew Chem. Int. Ed. Engl.53(31)8211-8215(2014) 7.Verhasselt, S., Roman, B.I., Bracke, M.E., et al.Improved synthesis and comparative analysis of the tool properties of new and existing D-ring modified (S)-blebbistatin analogsEur. J. Med. Chem.13685-103(2017)