包装: | 36 µg |
市场价: | 13650元 |
Canonical SMILES | O=[13C]1[15NH][13C]([15NH2])=[15N][13C]2=[13C]1[15N]=[13CH][15N]2[13C@@H]([13C@@H]3O)O[13C@@]([H])([13C@]3(OP([O-])(O[13CH2][13C@]([H])([13C@]4(O5)[H])O[13C@H]([13C@@H]4O)[15N]6[13C]([15N]=[13C]([15NH]7)[15NH2])=[13C]([15N]=[13CH]6)[13C]7=O)=O)[H])[13CH2]OP5([O-])=O.[Na+].[Na+] |
分子式 | [13C]20H22[15N]10O14P2 • 2Na |
分子量 | 764.2 |
溶解度 | Water: soluble |
储存条件 | -20°C |
General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while. |
Shipping Condition | Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request |
产品描述 | 13C20,15N10-Cyclic di-GMP is intended for use as an internal standard for the quantification of cyclic di-GMP by GC- or LC-MS. Cyclic di-GMP is a second messenger in bacteria involved in diverse prokaryotic processes, including biofilm formation, motility, virulence, and cell cycling.1,2In eukaryotic cells, cyclic di-GMP is detected by and binds to the transmembrane protein stimulator of interferon genes (STING; Kd= 1.21 µM), leading to activation of the innate immune system.3,4It has been used at a preset molar ratio with STING dimers in binding assays to determine the binding constants of particularly tight binding partners, such as 2’3’-cGAMP. Cyclic di-GMP induces IFN-β mRNA expressionin vitro(EC50= 537.8 nM) but less potently than 2’3’-cGAMP , 3’2’-cGAMP, 3’3’-cGAMP , and 2’2’-cGAMP . 1.RÖmling, U., Galperin, M.Y., and Gomelsky, M.Cyclic di-GMP: The first 25 years of a universal bacterial second messengerMicrobiol. Mol. Biol. Rev.77(1)1-52(2013) 2.MartÍnez, L.C., and Vadyvaloo, V.Mechanisms of post-transcriptional gene regulation in bacterial biofilmsFront. Cell. Neurosci.4(38)1-15(2014) 3.Schaap, P.Cyclic di-nucleotide signaling enters the eukaryote domainIUBMB Life65(11)897-903(2013) 4.Zhang, X., Shi, H., Wu, J., et al.Cyclic GMP-AMP containing mixed phosphodiester linkages is an endogenous high-affinity ligand for STINGMol. Cell51(2)226-235(2015) |