In vitro activity: ML324 exhibits good Caco-2 cell permeability, and possesses excellent microsomal stability in the presence of both mouse and rat liver microsomes. ML324 demonstrates potent anti-viral activity against both herpes simplex virus (HSV) and human cytomegalovirus (hCMV) infection by inhibiting viral IE gene expression.

Kinase Assay: ML324 is a potent and cell-permeable JMJD2 demethylase inhibitor (IC50 = 920 nM).

Cell Assay: ML324 acted as an antiviral agent that effectively inhibited HSV and hCMV IE gene expression in HFF and MRC-5 cells, resulted in suppression of HSV plaques formation and inhibition of HSV infection spread.

Probe Reports from the NIH Molecular Libraries Program;