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Hsf1 Monoclonal Antibody(Clone 10H8)
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Hsf1 Monoclonal Antibody(Clone 10H8)图片
包装与价格:
包装价格(元)
25 µg电议
100 µg电议

产品介绍
For immunochemical analysis of Hsf1
分子量95
储存条件-20°C
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping ConditionEvaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
产品描述

Heat shock factor 1 (Hsf1) belongs to a family of heat shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function.1The up-regulation of Hsp expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor.2,3,4Most Hsfs have highly conserved amino acid sequences. On all Hsfs there is a DNA binding domain at the N-terminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists and is thought to be necessary for suppression of trimerization.5There are two main Hsfs, 1 and 2. Murine Hsf1 exists as two isoforms, however in higher eukaryotes Hsf1 is found in a diffuse cytoplasmic and nuclear distribution in unstressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, Hsf1 dissipates from these granules to a diffuse nucleoplasmic distribution. Hsf2 on the other hand is similar to murine Hsf1, as it exists as two isoforms, the α form being more transcriptionally active than the smaller β form.6,7Various experiments have suggested that Hsf2 may have roles in differentiation and development.8,9,10

1.Morano, K.A., and Thiele, D.J.Heat shock factor function and regulation in response to cellular stress, growth, and differentiation signalsGene Expression7271-282(1998) 2.Tanaka, K.I., Namba, T., Arai, Y., et al.Genetic evidence for a protective role for heat shock factor 1 and heat shock protein 70 against colitisThe Journal of Biological Chemisty(2007) 3.Morimoto, R.I.Regulation of the heat shock transcriptional response: Cross talk between a family of heat shock factors, molecular chaperones, and negative regulatorsGenes & Development123788-3796(1998) 4.McMillan, D.R., Xiao, X., Shao, L., et al.Targeted disruption of heat shock transcription factor 1 abolishes thermotolerance and protection against heat-inducible apoptosisThe Journal of Biological Chemisty273(13)7523-7528(1998) 5.Jolly, C., Usson, Y., and Morimoto, R.I.Rapid and reversible relocalization of heat shock factor 1 within seconds to nuclear stress granulesProceedings of the National Academy of Sciences of the United States of America966769-6774(1999) 6.Fiorenza, M.T., Farkas, T., Dissing, M., et al.Complex expression of murine heat shock transcription factorsNucleic Acids Research23(3)467-474(1995) 7.Goodson, M.L., Park-Sarge, O.K., and Sarge, K.D.Tissue-dependent expression of heat shock factor 2 isoforms with distinct transcriptional activitesMolecular and Cellular Biology15(10)5288-5293(1995) 8.Rallu, M., Loones, M., Lallemand, Y., et al.Function and regulation of heat shock factor 2 during mouse embryogenesisProceedings of the National Academy of Sciences of the United States of America942392-2397(1997) 9.Sarge, K.D., Park-Sarge, O.K., Kirby, J.D., et al.Expression of heat shock factor 2 in mouse testis: Potential role as a regulator of heat-shock protein gene expression during spermatogenesisBiology of Reproduction501334-1343(1994) 10.Murphy, S.P., Gorzowski, J.J., Sarge, K.D., et al.Characterization of constitutive HSF2 DNA-binding activity in mouse embryonal carcinoma cellsMolecular and Cellular Biology14(8)5309-5317(1994)