Cell Counting Kit-8(CCK-8)

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Cell Counting Kit-8(CCK-8)

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

Cell Counting Kit-8(CCK-8)图片

包装与价格：

包装	价格(元)
1 x 5mL (500 T)	电议
2 x 5mL (1000 T)	电议
6 x 5mL (3000 T)	电议
10 x 5mL (5000 T)	电议
20 x 5mL (10000 T)	电议

产品介绍

Cell Counting Kit-8，简称CCK-8试剂盒或CCK8试剂盒，是一种基于WST-8而广泛应用于细胞活性和细胞毒性检测的快速、高灵敏度试剂盒。

应用&特点	1) 细胞增殖测定 - GlpBio细胞计数试剂盒-8（CCK-8）是水溶性的，在培养中是稳定的，并且是无毒的。 2) 细胞活力测定 - 代谢活性和染料产生与改变的活力成比例地变化。 3) 细胞因子测定 - 测量细胞因子诱导的增殖。如果需要，可以在研究结束时回收细胞并扩增。 4) 细胞毒性测定 - 来自细胞毒性化学物质的细胞死亡对颜色发展没有影响，只有活细胞将试剂转化为比色指示剂。试剂本身具有可忽略的毒性，并且通常对细胞是安全的。
运输方式	蓝冰运输。
储存条件	储存在4℃避光，可稳定长达12个月。储存在-20℃避光，可稳定长达2年。
用途	仅供研究使用!不能用于人体。
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产品描述	细胞计数试剂盒-8（CCK-8）允许使用WST-8（2-（2-甲氧基-4-硝基苯基）-3-（4-硝基苯基）-5-（2,4-二磺基苯基）-2H-）进行方便的分析四唑盐，一钠盐），其在电子载体1-甲氧基PMS存在下生物还原时产生水溶性甲dye染料。将CCK-8溶液直接添加到细胞中，不需要预先混合组分。 WST-8被细胞脱氢酶生物还原为可溶于组织培养基的橙色甲臜产物。产生的甲的量与活细胞的数量成正比。由于CCK-8溶液非常稳定并且几乎没有细胞毒性，因此可以进行更长的孵育，例如24至48小时。细胞计数试剂盒-8允许灵敏的比色测定法测定增殖和细胞毒性测定中活细胞的数量。检测灵敏度高于任何其他四唑盐，如MTT，XTT或MTS。图1: Cell Counting Kit-8 (CCK-8)的工作机制.