IBS008738 是一种有效的 TAZ 激活剂。IBS008738 稳定C2C12 细胞中的 TAZ，增加未磷酸化的 TAZ 水平，增强 MyoD 与肌细胞生成素启动子的结合，上调 MyoD 依赖性基因转录，并与肌肉生长抑制素竞争。IBS008738 增强 C2C12 细胞的肌生成并促进肌肉损伤模型中的肌肉修复。
| Cas No. | 385425-03-6 |
| 分子式 | C22H22N4O2 |
| 分子量 | 374.44 |
| 溶解度 | DMSO : 50 mg/mL (133.53 mM; Need ultrasonic) |
| 储存条件 | Store at -20°C |
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while. |
| Shipping Condition | Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request |
| 产品描述 | IBS008738 is a potentTAZactivator. IBS008738 stabilizes TAZ, increases the unphosphorylated TAZ level, enhances the association of MyoD with the myogenin promoter, upregulates MyoD-dependent gene transcription, and competes with myostatin in C2C12 cells. IBS008738 enhances myogenesis in C2C12 cells and facilitates muscle repair in a muscle injury model[1][2]. IBS008738 (0, 24, 48, 72 h) enhances TAZ expression, with a peak at 24 h[1].
IBS008738 (10 μM; 0, 24, 72 h) enhances mRNAs of myogenic markers but not of myofusion markers[1].
IBS008738 (10 μM; 24h) enhances mRNAs of IL-10 in C2C12 cells[2]. Western Blot Analysis[1] | Cell Line: | C2C12 cells | | Concentration: | | | Incubation Time: | Treated for 0, 24, 48, 72 h under differentiation conditions after 24 h under growth conditions. | | Result: | Enhanced TAZ expression, with a peak at 24 h. |
RT-PCR[1] | Cell Line: | C2C12 cells | | Concentration: | 10 μM | | Incubation Time: | 0, 24, 72 h under differentiation | | Result: | Enhanced mRNAs of myogenic markers but not of myofusion markers. |
IBS008738 (3 nmol) facilitates muscle repair in Cardiotoxin-injected muscles[1].
IBS008738 (30 μM, 100 μL) prevents Dexamethasone-induced muscle atrophy[1].
IBS008738 (100-μL volume of 30 μM; intramuscular injection; immediately and on day 2 after TBI) diminishes the expression of TNF α and IL-6 but increases that of IL-10 mRNAs in muscles of traumatic brain injury (TBI)[2]. | Animal Model: | Six-week-old female BALB/cByJ mice[1] | | Dosage: | 3 nmol (0.3 μL of 10 mM IBS008738 was diluted in 100 μL of PBS) | | Administration: | Injected into the tibialis anterior (TA) muscle of mice under anesthesia. | | Result: | Facilitated muscle repair in Cardiotoxin-injected muscles. |
| Animal Model: | Six-week-old female BALB/cByJ mice[1] | | Dosage: | 30 μM (100 μL) | | Administration: | Injected into the tibialis anterior (TA) and gastrocnemius (GM) muscles on days 9, 11, and 13. | | Result: | Prevented Dexamethasone-induced muscle atrophy. |
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