包装 | 价格(元) |
5mg | 电议 |
10mg | 电议 |
25mg | 电议 |
Cell lines | Murine RAW 264.7 macrophages |
Preparation Method | The prepared mouse bone marrow-derived macrophages were cultured and stored at 37℃ in 5% CO2. Murine RAW 264.7 macrophages were treated with EtOH (80 mM), MeCHO (200 μM), and nicotinamide riboside (1 mM), respectively, and ROS accumulation was determined. |
Reaction Conditions | 1 mM ,72 h |
Applications | Ethanol and its metabolite, acetaldehyde, significantly increased cellular ROS levels, but Nicotinamide riboside completely abolished the increase to a basal level in RAW 264.7 macrophages. |
Animal models | Eight-week-old male C57BL/6 J mice |
Preparation Method | The reared mice were randomly divided into 3 groups: control group (CTRL), ethanol group (EtOH) and nicotinamide riboside supplementation group (EtOH+ Nicotinamide riboside ). Mice in the ethanol and nicotinamide riboside supplemented groups were fed a Lieber-DeCarli ethanol liquid diet, while control mice were paired as previously described. |
Dosage form | 400 mg/kg, oral gavage once daily for 10 consecutive days |
Applications | Mice were fed in pairs and there was no difference in body weight between the three groups. Fat accumulation was observed in the ethanol group, while only a few tiny lipid droplets were observed in the nicotinamide riboside group. Nicotinamide riboside significantly decreased serum ALT and AST and liver triglyceride levels, and slightly decreased liver weight ratio. |
产品描述 | Nicotinamide riboside, a form of vitamin B3 and NAD+ precursor, is converted to bioavailable NAD+, via nicotinamide riboside kinase (NRK) and NMNAT, or by the action of nucleoside phosphorylase and NAM salvage[1-2]. In in vitro experiments ,nicotinamide riboside attenuates alcohol induced liver injuries via activation of SirT1/PGC-1α/mitochondrial biosynthesis pathway[3]. Nicotinamide Riboside Enhances In Vitro Beta-adrenergic Brown Adipose Tissue Activity in Humans[4]. Nicotinamide Riboside was able to enhance the skeletal muscle NAD+ metabolome, inducing gene expression signatures implicated in downregulation of energy metabolism pathways, but did not affect muscle mitochondrial bioenergetics or metabolism[5]. Nicotinamide Riboside enhances deacetylase activity in vivo, deacetylates PGC-1α in muscle, liver, and BAT, and it induces deacetylase activity in tissues where NAD+ accumulates[6]. References: |