| 包装 | 价格(元) |
| 10mM (in 1mL DMSO) | 电议 |
| 10mg | 电议 |
| 25mg | 电议 |
| 50mg | 电议 |
| 100mg | 电议 |
| 200mg | 电议 |
| 500mg | 电议 |
| 1g | 电议 |
| 2g | 电议 |
| 5g | 电议 |
Cell lines | HGF-Treated Lens Epithelial Cells (LECs) |
Preparation Method | After pretreatment with HGF for 12 h, LEC was treated with increased doses of rapamycin (0, 1.5 and 10 ng/mL) at different time points (24, 48 and 72 h). |
Reaction Conditions | 1/5/10 ng/mL for 24/48h/72h |
Applications | Rapamycin inhibited the proliferation of lens epithelial cells (LEC) induced by hepatocyte growth factor (HGF) in a dose-and time-dependent manner. |
Animal models | Ndufs4 -- / -- mice |
Preparation Method | Rapamycin (8 mg/kg) was delivered by intraperitoneal injection every other day from weaning [approximately day 20 postpartum (P20)]. |
Dosage form | 8 mg/kg, every other day, by intraperitoneal injection |
Applications | Rapamycin reduces neurologic disease in Ndufs4 -- / -- mice. |
| 文献引用 | |
| 产品描述 | Rapamycin used to be used as an antifungal antibiotic[3]. Rapamycin exerts immunosuppressive effects by inhibiting the activation and proliferation of T cells. Rapamycin binds to FK-binding protein 12 (FKBP12) to form the Rapamycin-FKBP12 complex, which can inhibit mTOR[4,6].As a potent and specific mTOR inhibitor with an50of 0.1 nM in HEK293 cells. Rapamycin binds to FKBP12 and specifically acts as an allosteric inhibitor of mTORC1[5]. Rapamycin (12.5-100 nM; 24 hours) treatment exerts modest inhibitory effect on lung cancer cell proliferation in a dose-dependent manner in all cell lines (A549, SPC-A-1, 95D and NCI-H446 cells) tested, achieving about 30-40% reduction in cell proliferation at 100 nM vs. ~10% reduction at 12.5 nM[7].Rapamycin potently not only suppressed proliferation but also induced the apoptosis of LECs in a dose-dependent manner under HGF administration. Rapamycin could promote apoptosis of LECs via inhibiting HGF-induced phosphorylation of AKT/mTOR, ERK and JAK2/STAT3 signaling molecules[1]. Rapamycin reduces neurologic disease in Ndufs4 -- / -- mice. In rapamycin treated knockout mice, the percentage of mice exhibiting neurological symptoms was greatly reduced at each age point after P35, and about half of these mice never showed obvious signs of neurological disease before dying[2]. Rapamycin alone has a moderate inhibitory effect. However, the combination of Metformin and Rapamycin exerts a significantly increased inhibition of tumor growth compared with the control group, the Rapamycin monotherapy group and the Metformin monotherapy group[8].Rapamycin treatment in cell culture significantly inhibits c-Myc-regulated gene expression. Rapamycin suppresses tumor growth along with a decreased expression of STAT3 and c-Myc in an in vivo xenograft mouse model for hepatocellular carcinoma[9]. References: |
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