3,6-二乙酰氧基邻苯二甲腈是一种可渗透细胞的荧光探针。
Cas No. | 83619-73-2 |
别名 | 1,4-二乙酰基-2,3-二氰苯 |
Canonical SMILES | CC(OC1=CC=C(OC(C)=O)C(C#N)=C1C#N)=O |
分子式 | C12H8N2O4 |
分子量 | 244.2 |
溶解度 | DMF: 30 mg/ml,DMSO: 30 mg/ml,DMSO:PBS(pH7.2) (1:1): 0.5 mg/ml |
储存条件 | Store at -20°C |
General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while. |
Shipping Condition | Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request |
产品描述 | 3,6-diacetoxy Phthalonitrile is a fluorescent probe for monitoring intracellular pH by flow cytometry in living cells. It rapidly crosses cell membranes and is cleaved by cytosolic esterases to form the fluorescent pH indicator 2,3-dicyano-hydroquinone (DCH), which is excited at UV wavelengths. DCH can be excited at 351 nm, with pH-dependent emission in the range of 450-476 nm (pH 5.0-10.0) using a potassium-based buffer. |