您好，欢迎来到化工原料网！ [登录] [免费注册]
化工原料网
发布求购
位置：首页 > 产品库 > Leishman's stain
立即咨询
咨询类型：
     
*姓名：
*电话：
*单位：
Email：
*留言内容：
请详细说明您的需求。
*验证码：
 
Leishman's stain
本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
CAS NO:12627-53-1
包装:100g
市场价:1069元

产品介绍
Leishman's stain 是用于对染外周血和骨髓涂片进行染色的重要工具 (在油镜下呈淡蓝灰色至深蓝色)。
Cas No.12627-53-1
别名瑞特染色剂
储存条件Store at -20°C
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping ConditionEvaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
产品描述

Leishman's stain is an essential staining tool for for staining of the peripheral blood and bone marrow smears (displayed pale bluish-grey to deep blue under oil-immersion lens)[1].

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1].
1. Prepare stock solution of Sorensen's buffer: Solution A. Na2HPO4•2H2O (9.5 g/L); Solution B. KH2PO4(9.1 g/L).
2. Cover the smear with Leishman stain for 2 min.
3. Add double volume of Sorensen buffer (6.8 pH) to the slide and mix gently for 15 min.
4. Wash off with running water and dry the smear (5 min).

[1]. S Gajendra, et al. Leishman and Giemsa stain: a new reliable staining technique for blood/bone marrow smears. Int J Lab Hematol. 2015 Dec;37(6):774-82.