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Tubacin
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Tubacin图片
CAS NO:537049-40-4
包装:2mg, 5mg, 10mg
包装与价格:
包装价格(元)
2mg电议
5mg电议
10mg电议

产品名称
Tubulin Acetylation Inducer
产品介绍

生物活性

Tubacin是一种高效的,选择性的,可逆的,细胞通透性的HDAC6抑制剂,IC50为4 nM。


化学数据

分子量721.86
分子式C41H43N3O7S
CAS号537049-40-4
纯度100.00%
溶解性(25°C)DMSO 90 mg/mL
储存和运输条件固体粉末: -20°C 冷藏长期储存
常温运输及临时存放

实验操作 来自于公开的文献,仅供相同实验参考(如实验材料、目的不同,请参考其他文献)

细胞实验
细胞系ALL cells and normal T lymphocytes
方法Growth inhibition assay The inhibitory effect of tubacin on ALL cells and normal T lymphocytes was assessed by colorimetric assay, 3-(4,5-dimethylthiazol-e-yl)- 2,5-diphenyl tetrazolium bromide (MTT; Sigma). Only active mitochondria dehydrogenase enzymes can perform cleavage of a tetrazolium ring, and therefore the amount of purple formazan generated by cells with a drug was compared with the amount of formazan produced from non-treated cells. Absorbance readings at a wavelength of 550 nm were taken on a spectrophotometer (Titertek Multiskan MCC Type 341). Cells from 72 h cultures were incubated with 10 μL of 5 mg/mL MTT for 2 h, followed by 100 μL isopropanol that contained 0.1 N HCl. Normal T lymphocytes were incubated with MTT for 30–37 min. All experiments were performed in triplicate.
浓度0, 0.5, 1, 1.5, 2, 2.5μM
处理时间72 h

动物实验
动物模型Xenograft system of primary leukemia samples
配制saline
剂量50 mg/kg/day
给药处理intraperitoneally

不同实验动物依据体表面积的等效剂量转换表(数据来源于FDA指南)

小鼠大鼠豚鼠仓鼠
重量 (kg)0.020.151.80.40.0810
体表面积 (m2)0.0070.0250.150.050.020.5
Km系数36128520
动物 A (mg/kg) = 动物 B (mg/kg) × 动物 B的Km系数
动物 A的Km系数

例如,依据体表面积折算法,将化合物用于小鼠的剂量20 mg/kg 换算成大鼠的剂量,需要将20 mg/kg 乘以小鼠的Km系数(3),再除以大鼠的Km系数(6),得到化合物用于大鼠的等效剂量为10 mg/kg。


储备液配制

以下数据基于产品分子量,对于特殊产品,请参照COA中的储备液配制条件和说明进行操作。

Concentration / Solvent Volume / Mass1 mg5 mg10 mg
1 mM1.3853 mL6.9266 mL13.8531 mL
5 mM0.2771 mL1.3853 mL2.7706 mL
10 mM0.1385 mL0.6927 mL1.3853 mL