Methiocarb-d₃is intended for use as an internal standard for the quantification of methiocarb by GC- or LC-MS. Methiocarb is a carbamate pesticide with insecticidal, molluscicidal, acaricidal, and bird repellant properties.^1,2,3,4,5It inhibits acetylcholinesterase (AChE) activity and is toxic to the western flower thrip (F. occidentalis; IC₅₀= 2.1 μM; LC₅₀= 3.26 ppm).²Methiocarb is also toxic to the land snailM. obstructa(LD₅₀= 27.4 μg/snail) and the citrus miteA. pelekassi, inducing 94% mortality when used at a concentration of 0.5 ppm.^1,3It reduces the seasonal percentages of cotton bolls infested with pink bollworm (P. gossypiella) to 4%, compared with 65% infestation in untreated controls, when applied to cotton fields at a concentration of 1.86 lb/acre.⁵Methiocarb reduces damage to grapes by song birds when applied to vineyards at a concentration of 2.5 lbs AI per 100 gal water per acre.⁴It is an estrogen receptor α (ERα) and ERβ agonist (EC₂₀s = 19.87 and 20.24 μM, respectively) and an androgen receptor antagonist (IC₅₀= 13.89 μM) in cell-based reporter assays.⁶It is toxic to juvenile rainbow trout (O. mykiss) and guppies (P. reticulata) with LC₅₀values of 0.8 and 1.48 mg/L, respectively, at 48 hours of exposure.⁷Formulations containing methiocarb have been used in the agricultural control of pests.

1.Hussein, H.I., Al-Rajhy, D., El-Shahawi, F.I., et al.Molluscicidal activity of Pergularia tomentosa (L.), methomyl and methiocarb, against land snailsInt. J. Pest Mgt.45(3)211-213(2010) 2.Jensen, S.E.Acetylcholinesterase activity associated with methiocarb resistance in a strain of western flower thrips, Frankliniella occidentalis (Pergande)Pesicide Biochem. Physiol.61(3)191-200(1998) 3.Reed, D.K., Crittenden, C.R., and Lyon, D.J.Acaricides screened against two rust mites of citrusJ. Econ. Entomol.60(3)668-671(1967) 4.Kassa, H., and Jackson, W.B.Mesurol as a bird repellent on grapes in OhioBird Control Seminars Proceedings(1979) 5.McGarr, R.L., Chapman, A.J., and Martin, D.F.Field tests with several insecticides for control of the pink bollworm, boll weevil, and bollwormJ. Econ. Entomol58(4)693-694(1965) 6.Tange, S., Fujimoto, N., Uramaru, N., et al.In vitro metabolism of methiocarb and carbaryl in rats, and its effect on their estrogenic and antiandrogenic activitiesEnviron. Toxicol. Pharmacol.41289-297(2016) 7.Boran, M., Altinok, I., and Capkin, E.Acute toxicity of carbaryl, methiocarb, and carbosulfan to the rainbow trout (Oncorhynchus mykiss) and guppy (Poecilia reticulata)Turk. J. Vet. Animal Sci.31(1)39-45(2007)