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Ac-VDVAD-AFC
本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
Ac-VDVAD-AFC图片
CAS NO:210344-94-8
包装与价格：
包装价格(元)
1mg电议
5mg电议
10mg电议

产品介绍
Ac-VDVAD-AFC 是一种半胱天冬酶特异性荧光底物。 Ac-VDVAD-AFC可测定caspase-3样活性和caspase-2活性，可用于肿瘤和癌症的研究。
Cas No.210344-94-8
别名N-Acetyl-Val-Asp-Val-Ala-Asp-7-amino-4-Trifluoromethylcoumarin Caspase-2 Substrate (Fluorogenic)
化学名N-acetyl-L-valyl-L-α-aspartyl-L-valyl-L-alanyl-N-[2-oxo-4-(trifluoromethyl)-2H-1-benzopyran-7-yl]-L-α-asparagine
Canonical SMILESO=C(O)CC(NC(C(NC(C(NC(C(NC(C(NC(C)=O)C(C)C)=O)CC(O)=O)=O)C(C)C)=O)C)=O)C(NC1=CC(O2)=C(C=C1)C(C(F)(F)F)=CC2=O)=O
分子式C33H41F3N6O12
分子量770.71
溶解度DMF: 10 mg/ml,DMSO: 10 mg/ml
储存条件Store at -20℃
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping ConditionEvaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
产品描述

Ac-VDVAD-AFC is a fluorogenic substrate whose amino acids VDVAD have been shown to be a preferred cleavage site for caspase-2.1,2 Caspase activity can be quantified by fluorescent detection of free AFC (also known as 7-amino-4-trifluoromethylcoumarin), which is excited at 400 nm and emits at 505 nm. Increases in Ac-VDVAD-AFC cleavage have been reported to correlate with nitric oxide-induced activation of caspase-2 and subsequent apoptosis in human neuroblastoma lines.1

Product Description References
1. Moriya, R., Uehara, T., and Nomura, Y. Mechanism of nitric oxide-induced apoptosis in human neuroblastoma SH-SY5Y cells. FEBS Lett. 484(3), 253-260 (2000).

2. Talanian, R.V., Quinlan, C., Trautz, S., et al. Substrate specificities of caspase family proteases. The Journal of Biological Chemisty 272(15), 9677-9682 (1997).