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3-(4-Pyridyl)indole
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
3-(4-Pyridyl)indole图片
CAS NO:7272-84-6
包装与价格:
包装价格(元)
5mg电议
10mg电议
25mg电议
50mg电议

产品介绍
3-(4-Pyridyl)indole (Rockout) 是一种 Rho 激酶 (ROCK) 抑制剂,IC50 为 25 μM。
Cas No.7272-84-6
别名Rho Kinase Inhibitor III,ROCK Inhibitor III,Rockout
化学名3-(4-pyridinyl)-1H-indole
Canonical SMILESC12=CC=CC=C1NC=C2C3=CC=NC=C3
分子式C13H10N2
分子量194.2
溶解度≤30mg/ml in ethanol;20mg/ml in DMSO;30mg/ml in dimethyl formamide
储存条件Store at -20℃
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping ConditionEvaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
产品描述

3-(4-pyridyl)indole (Rockout) is a Rho-kinase inhibitor [1].

Rho-associated protein kinase (ROCK) belongs to a family of serine-threonine kinases mainly involved in regulating the shape and movement of cells by acting on the cytoskeleton [2].

Rockout caused decreased migration and aberrant morphology. Rockout showed no effect on phagocytosis, listeria motility or actin polymerization in permeabilized yeast. In a human melanoma cell line M2 cells that blebs constitutively in a manner that is sensitive to actin cytoskeletal perturbation, Rockout (50 μM) stopped the cell blebbing within 2 min and with washout blebbing returned to normal levels within 3 min. The IC50 of Rockout was 12 μM and it inhibited blebbing in nearly all cells at 50 μM. In B3T3 cells, treatment with 50 μM Rockout resulted in significant morphological change within 10 min [1]. At an ATP concentration of 100 μM, Rockout inhibited ROCK activity with an approximate IC50 of 25 μM. Rockout (25 μM) inhibited ROCK-II and PRK to a similar degree, and MSK-1 and PKA to a lesser extent. Rockout showed no inhibitory effect on PKCα or SAPK2a [1].

References:
[1] Yarrow J C, Totsukawa G, Charras G T, et al.  Screening for cell migration inhibitors via automated microscopy reveals a Rho-kinase inhibitor[J]. Chemistry & biology, 2005, 12(3): 385-395.
[2] Amano M, Fukata Y, Kaibuchi K.  Regulation and functions of Rho-associated kinase[J]. Experimental cell research, 2000, 261(1): 44-51.