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Lomustine
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Lomustine图片
包装与价格:
包装价格(元)
10mM (in 1mL DMSO)电议
250mg电议
500mg电议

产品介绍
Lomustine (CCNU; NSC 79037) 是一种 DNA 烷化剂,具有抗肿瘤活性。

Cell lines

L1210 leukemia cells

Preparation method

The solubility of this compound in DMSO is >11.7mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.

Reacting condition

50 μg/ml, 37℃

Applications

In L1210 leukemia cells, the radioactivity of the cyclohexyl moiety of CCNU (Lomustine) was bound almost exclusively to proteins, and that of the ethylene group was bound (at a much lower level) to both nucleic acids and proteins.

Animal models

L1210 leukemia-bearing mice

Dosage form

0.5 mg in 0.2 ml of dimethyl sulfoxide; i.p. injection

Application

In L1210 leukemia-bearing mice, the radioactivity of the cyclohexyl moiety of CCNU (Lomustine) was almost exclusively bound to cellular proteins, rather than to nucleic acids. The radioactivity of the ethylene moiety was bound to both nucleic acids and proteins of brain, liver, and leukemia cells to about the same level.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

产品描述

Lomustine is an antineoplastic drug used in chemotherapy [1]

Lomustine has been revealed to inhibit the growth of tumour cell lines with IC50 values of 25μM, 8.8μM and 13μM for breast ZR-75-1, astrocytoma U87MG and colorectal LS174T cell lines [2]. Besides, Lomustine has been found to be particularly effective in the treatment of certain neoplasms of the central nervous system, because of the high lipid solubility and permeability through the blood brain barrier. In addition, Lomustine has shown the effect function in treatment of meningeal leukemia in the mouse and in children who have acute leukemia with central nervous system involvement [1].

References:
[1] Cheng CJ, Fujimura S, Grunberger D, Weinstein IB. nteraction of 1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea (NSC 79037) with nucleic acids and proteins in vivo and in vitro. Cancer Res. 1972 Jan;32(1):22-7.
[2] Baer JC1, Freeman AA, Newlands ES, Watson AJ, Rafferty JA, Margison GP. Depletion of O6-alkylguanine-DNA alkyltransferase correlates with potentiation of temozolomide and CCNU toxicity in human tumour cells.Br J Cancer. 1993 Jun; 67(6):1299-302.