| 包装 | 价格(元) |
| 10mM (in 1mL DMSO) | 电议 |
| 20mg | 电议 |
Cell experiment: | Cytotoxic effects of Betaine hydrochloride are evaluated by MTT assays. Briefly, 5×103 HeLa or MCF-10A cells/well are seeded in a 96-well plate and cultured overnight. Then, different concentrations of Betaine hydrochloride (from 0 to 100 mg/mL) are added to each well, and the MTT assay is performed at 24, 48, 72 and 96 h after incubation. Cu/Zn-SOD activities are detected using the superoxide dismutase Assay Kit according to the manufacturer’s instructions[1]. |
Animal experiment: | Male C57BL/6 mice weighing 25±0.5 g are used in this study. Animals are randomly assigned to four groups (n=6 to 8 per group) and pair-fed a modified Lieber-DeCarli alcohol (AF) or isocaloric maltose dextrin control liquid diet (PF) for 5 weeks with a stepwise feeding procedure. The ethanol content starts at 30% of total calories and gradually increases up to 36% in the last week. Betaine hydrochloride is administered as a supplement in the liquid diets (both PF and AF) at a concentration of 0.5% (w/v), simultaneously with the alcohol. All animals have access to diet and water ad libitum. At the end of the experiment, the mice are killed after being deprived of food for 4 h, and the plasma, liver and epididymal fat pad samples are harvested for assays[2]. |
| 产品描述 | Betaine hydrochloride is a natural compound found in many foods and also an active methyl-donor which can maintain normal DNA methylation patterns. It is found that proliferation of HeLa cells is inhibited significantly upon exposure to increasing Betaine hydrochloride levels with the MTT test (p5.0 mg/mL) significantly promotes the apoptosis of HeLa cells (p<0.01). SOD activities of the low dose groups are slightly higher than the control group (p<0.05), and there are obvious synchronicity and correlation among the expression of promoting apoptosis genes Bax, P53, Caspase 3 and apoptosis suppression gene Bcl-2. In response to an apoptosis-inducing stimulus, p53 and cyclin D1 can be activated with blockage of the cell cycle at G1/S or S/G2 checkpoints[1]. Liver-to-body weight ratio is attenuated by Betaine hydrochloride supplementation. When Betaine hydrochloride is added to the alcohol-containing liquid diet, both hepatic triglyceride (TG) accumulation and liver injury are significantly reduced. Betaine hydrochloride supplementation attenuates epididymal fat pad mass loss induced by chronic alcohol feeding. Betaine hydrochloride supplementation prevents the depletion of S-adenosylhomocysteine (SAM) and reduces the increase in S-adenosylhomocysteine (SAH), thereby improving the methylation state of the adipose tissue[2]. References: |
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