| 包装 | 价格(元) |
| 500μg | 电议 |
| 1mg | 电议 |
| 5mg | 电议 |
| 10mg | 电议 |
Cell lines | Human primary fibroblasts |
Preparation Method | NPC1‐deficient GM3123 fibroblasts were incubated with Rhodamine‐dextran overnight to label terminal endocytic compartments, followed by incubation with 5 μg/mL (8.7 μm) BODIPY-Cholesterol in normal growth medium containing 5% fetal calf serum for 4 h, or 0.5 μm BODIPY-Cholesterol in medium containing 5% lipoprotein‐starved serum for 23 h. |
Reaction Conditions | 5 μg/mL, 4h |
Applications | Time‐dependent partitioning of BODIPY-Cholesterol in the lysosomes of human primary fibroblasts. |
Cell lines | NIH 3T3 |
Preparation Method | NIH 3T3 were cultured in 24-well plates, incubated for 2 h with 2.5 μM BODIPY-cholesterol, and rinsed. Physiological buffer with or without 30 nM ShhN was added to the wells, and the BODIPY fluorescence intensity of the supernatants was measured after 1 h. |
Reaction Conditions | 2.5 μM; 2 h |
Applications | The BODIPY fluorescence measured in the supernatants after 1 h was significantly lower in wells where ShhN protein was added compared to wells that did not contain ShhN. |
| 文献引用 | |
| 产品描述 | BODIPY-Cholesterol is cholesterol tagged with a boron dipyrromethene difluoride (BODIPY) fluorophore used for monitoring sterol uptake and inter-organelle sterol flux in cells with excitation of 480 nm and emission of 508 nm.[1]The excretion of BODIPY cholesterol from late endoplasmic organelles depends on acidic lipase and Niemann pickc1 protein.[6] In vitro, treatment with Bodipy-cholesterol in cells, prominent PM labeling is observed at 2–5 min; however, upon ≥30 min incubations, it is also observed the fluorescence labeling of intracellular structures.[2]In vitro efficacy test it suggested that accumulation of BODIPY-cholesterol in the media gave more reproducible values than assaying for the loss of the compound from the cells.[3]With 1 μg BODIPY-cholesterol analogs show a similar cellular localization in HeLa cells and exhibit similar cholesterol efflux properties from THP1 cells to HDL acceptors.[4]BODIPY-cholesterol efflux clearly increased when treatment of fibroblasts with the Hh pathway agonist SAG, which enhances Ptc protein expression, or over-expression of human Ptc in yeast.[5]Treatment with miR-758 inhibitor obviously increased ABCA1-dependent cholesterol efflux by BODIPY-Cholesterol efflux assay.[7]Micrographs of EPCs incubated with HDL labeld bodipy-cholesterol (50 μg/ml) 30 min, small cytoplasmic vesicles as well as large positive MVBs containing intraluminal microvesicles, tightly-packed with reaction products could be displayed. The internalized-HDL-derived bodipy-cholesterol was also spread within many of the stacked Golgi cisterns and the TGN.[8] References: |
m.cnreagent.com