包装 | 价格(元) |
10mM (in 1mL DMSO) | 电议 |
100mg | 电议 |
Cell lines | COLO 357 and L3.6pl cells |
Preparation method | The solubility of this compound in DMSO is<10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37 ℃ for 10 minutes and/or shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. |
Reacting condition | 25 nM, 72 hours |
Applications | Cells were either pretreated with genistein (25 μmol/L) alone or in combination with a single dose of gemcitabine (25 nmol/L), and viable cells were evaluated at 96 hours posttreatment by MTT assay. Treatment with either genistein or gemcitabine alone for 96 hours resulted in only 25% to 30% loss of viability of COLO 357 and L3.6pl cells. However, pretreatment with genistein for 24 hours followed by treatment with gemcitabine resulted in the loss of 60% to 80% of viable cells in both the cell types investigated. These results suggests that the combination of genistein with low therapeutic doses of gemcitabine elicits significantly greater inhibition of cancer cell growth compared with either agent, suggesting that lower toxic side effects are likely to occur in normal cells. |
Animal models | Female nude mice (ICR-SCID) injected with COLO 357 or L3.6pl cells |
Dosage form | Intravenous injection, 80 mg/kg body weight, every other day for a total of three injections |
Application | Mice were randomized into the following treatment groups (n = 7): (a) untreated control; (b) only gemcitabine; (c) genistein, 1 mg genistein/d/mouse, everyday orally for 10 days; and (d) genistein and gemcitabine. Single modality treatment with either genistein or gemcitabine alone in mice harboring COLO 357 cells caused 13% and 27% reduction in tumor weight, respectively, compared with control tumors. Under identical experimental conditions, combination of genistein and gemcitabine treatment showed significant decrease in tumor weight compared with untreated control, genistein alone–, or gemcitabine alone– treated group. |
Other notes | Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
产品描述 | Gemcitabine HCl is an inhibitor of DNA synthesis with the IC50 value of 240.4±29.0 μM (CCRF-CEM/dCK−/− cells), 14.7±2.8 nM (TC-1 cells), 36.7 ± 5.1 μM (TC-1-GR cells), and 50 nM (PANC1 cells) [1]. |