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Recombinant Rat EGF Protein
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Recombinant Rat EGF Protein图片
包装与价格:
包装价格(元)
100ug电议
1g电议

产品名称
Epidermal Growth Factor
产品介绍

生物活性

Recombinant Rat EGF Protein is type Itransmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.

Determined by BALB/c 3T3 cell proliferation analysis, its ED50 value is ≤ 0.1 ng/ml, and the corresponding specific activity is ≥ 1 x 107IU/mg.

Expression host is Human HEK293 cells


化学数据

分子量
分子式
CAS号
纯度≥95%, Endotoxin<0.1EU/ug
溶解性(25°C)Water
Saline
PBS
储存和运输条件2-8°C, dry, sealed
常温运输及临时存放

不同实验动物依据体表面积的等效剂量转换表(数据来源于FDA指南)

小鼠大鼠豚鼠仓鼠
重量 (kg)0.020.151.80.40.0810
体表面积 (m2)0.0070.0250.150.050.020.5
Km系数36128520
动物 A (mg/kg) = 动物 B (mg/kg) × 动物 B的Km系数
动物 A的Km系数

例如,依据体表面积折算法,将化合物用于小鼠的剂量20 mg/kg 换算成大鼠的剂量,需要将20 mg/kg 乘以小鼠的Km系数(3),再除以大鼠的Km系数(6),得到化合物用于大鼠的等效剂量为10 mg/kg。