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Procyanidin C1
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Procyanidin C1图片
包装与价格:
包装价格(元)
10mM (in 1mL DMSO)电议
1mg电议
5mg电议
10mg电议

产品介绍
原花青素 C1 (PCC1) 是一种天然多酚,可导致 DNA 损伤、细胞周期停滞并诱导细胞凋亡。 Procyanidin C1 降低 Bcl-2 的水平,但增强 BAX、caspase 3 和 9 在癌细胞中的表达。

Cell lines

RAW 264.7 macrophage cells and Bone marrow derived macrophages(BMDMs)

Preparation Method

RAW 264.7 macrophage cells (1×105cells/well) and BMDMs (0.5×106cells/well) were cultured in 48-well and 12-well plates with DMEM containing 10% FBS, and then incubated at 37℃ under 5% CO2 in an incubator until they reached 95% confluence. Procyanidin C1 (at concentrations of 31.25 and 62.5μg/ml) was added to each well and incubated at 37℃ for 24h.

Reaction Conditions

31.25μg/ml and 62.5μg/ml

Applications

Procyanidin C1 significantly (P

Animal models

Male ICR mice weighing 35–40g

Preparation Method

Male ICR mice were treated with a single oral dose of flavan3-ols(FL), epicatechin(EC), procyanidin B2(B2), procyanidin C1(C1), cinnamtannin A2(A2), or pentamer fraction(P5). The animals were sacrificed and blood and brown adipose tissue (BAT) sampled. The plasma catecholamine (CA) levels and BAT uncoupling protein (UCP)-1 mRNA expression were determined.

Applications

A single dose of 10mg/kg FL significantly increased plasma CA and UCP-1 mRNA levels. B2, procyanidin C1, and A2, but not EC and P5 (all at 1mg/kg), significantly increased plasma adrenaline levels. Plasma noradrenaline was significantly elevated by B2 and A2, but not by EC, procyanidin C1, or P5. UCP-1 mRNA levels were significantly increased by procyanidin C1 and P5. In the dose response study of A2, 10-3mg/kg A2 increased UCP-1 mRNA levels significantly, but not 10-2and 10-1mg/kg A2. In addition, combination treatment with 10-1mg/kg A2 and yohimbine, an α2 adrenalin blocker, remarkably increased UCP-1 mRNA levels.

产品描述

Procyanidin C1 is a polyphenolic compound, it is found in a variety of vegetables and fruits and has a wide range of biological activities, including antioxidant and anti-inflammatory anticancer roles[1,2]

Procyanidin C is a catechin trimer purified from Cinnamomi Cortex and it showed inhibitory activity against TGF-β-induced EMT[3]. Treatment with procyanidin C1 in BMDMs resulted in a significant decrease in prostaglandin E2 and cyclooxygenase-2 levels, as well as the expression of cell surface molecules (CD80, CD86, and MHC class II), which was induced by LPS[1]. procyanidin C1 induced cell cycle arrest at S-phase and activated check point kinases, Chk1 and 2 in both MCF-7 and MDA-MB- 231 cells. At 48 h treatment procyanidin C1 induced DNA damage. In addition, procyanidin C1 decreased the Bcl2 levels and increased the BAX levels in both MCF-7 and MDA-MB- 231cells, which indicate that the procyanidin C1 inhibits breast cancer cell growth by inhibiting proliferation and by inducing apoptosis[2]

Procyanidin C1, a polyphenolic component of grape seed extract, increases the healthspan and lifespan of mice through its action on senescent cells[4]. In vivo, zebrafish larvae (AB strain) 3 days post-fertilization were incubated with NAC or procyanidins (C, EC, ECG, B1, B2, B3, B4, B1-G, B2-G, C1) in 300 μM H2O2for 4 days. Different grape seed procyanidins increased the survival of PC12 cells challenged with H2O2, improved the movement behavior disorder of zebrafish caused by H2O2, inhibited the increase of ROS and MDA and the decrease of GSH-Px, CAT, and SOD activities, and up-regulated the Nrf2/ARE pathway. The neuroprotective effects of the procyanidin trimer C1 treatment group were greater than the other treatment groups[5]

References:
[1].Byun EB, Sung NY, et al. The procyanidin trimer C1 inhibits LPS-induced MAPK and NF-κB signaling through TLR4 in macrophages. Int Immunopharmacol. 2013;15(2):450-456.
[2].Koteswari LL, Kumari S, et al. A comparative anticancer study on procyanidin C1 against receptor positive and receptor negative breast cancer. Nat Prod Res. 2020;34(22):3267-3274.
[3].Kin R, Kato S, et al. Procyanidin C1 from Cinnamomi Cortex inhibits TGF-β-induced epithelial-to-mesenchymal transition in the A549 lung cancer cell line. Int J Oncol. 2013;43(6):1901-1906.
[4].Xu Q, Fu Q, Li Z, et al. The flavonoid procyanidin C1 has senotherapeutic activity and increases lifespan in mice. Nat Metab. 2021;3(12):1706-1726.
[5].Nakano N, Nishiyama C, Tokura T, et al. Procyanidin C1 from apple extracts inhibits Fc epsilon RI-mediated mast cell activation. Int Arch Allergy Immunol. 2008;147(3):213-221.