PAF C-16-d₄contains four deuterium atoms at the 7, 7', 8, and 8' positions of the hexadecyl moiety. It is intended for use as an internal standard for the quantification of PAF C-16 by GC-or LC-mass spectrometry. PAF C-16 is a naturally occurring phospholipid produced upon stimulation through two distinct pathways known as the "remodeling" and 'de novo' pathways.¹It is a potent mediator of neutrophil migration,²the production of reactive oxygen species,³and IL-6⁴in human macrophages. It is a more potent mediator of platelet aggregation than PAF C-18⁵. Pathological processes involving PAF include necrotizing enterocolitis,⁶inflammation, asthma, and allergy.⁷

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