N1-Methylpseudouridine

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CAS NO:

13860-38-3

包装与价格：

包装	价格(元)
10mg	电议
50mg	电议

产品介绍

化学性质

Physical Appearance	Solution
Storage	Store at -20°C or below
M.Wt	258.23
Cas No.	13860-38-3
Formula	C₁₀H₁₄N₂O₆
Solubility	≥50 mg/mL in H2O with ultrasonic; ≥20 mg/mL in EtOH; ≥20.65 mg/mL in DMSO
Chemical Name	5-((2S,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-1-methylpyrimidine-2,4(1H,3H)-dione
Canonical SMILES	O=C(N1)N(C)C=C([C@@H]2O[C@H](CO)[C@@H](O)[C@H]2O)C1=O
运输条件	蓝冰运输或根据您的需求运输。
一般建议	为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。

资料参考

N1-Methylpseudouridine is a modified nucleoside used for enhancing mRNA translation. Incorporation of N1-Methylpseudouridine into mRNA turns off the immune/eIF2α phosphorylation-dependent inhibition of translation, and increases ribosome pausing and density on the mRNA, thus dramatically facilitating the translation process. N1-Methylpseudouridine outperforms several other modified nucleosides (e.g. 5-Methylcytidine) and corresponding combinations in terms of translation capacity.

References:

1. Svitkin YV, Cheng YM, Chakraborty T, et al. N1-methyl-pseudouridine in mRNA enhances translation through eIF2α-dependent and independent mechanisms by increasing ribosome density. Nucleic Acids Research, 2017, 45(10): 6023-6036.

2. Andries O, Mc Cafferty S, De Smedt SC, et al N(1)-methylpseudouridine-incorporated mRNA outperforms pseudouridine-incorporated mRNA by providing enhanced protein expression and reduced immunogenicity in mammalian cell lines and mice. Journal of Controlled Release, 2015, 217: 337-344.

试验操作

Cell experiment:[2]
Cell lines	Various mammalian cells including A549, BJ, C2C12, HeLa and primary keratinocytes
Reaction Conditions	24 h incubation
Applications	In various mammalian cells, mRNAs in the N1-Methylpseudouridine + 5-Methylcytidine group showed reduced cytotoxicity compared to the Pseudouridine + 5-Methylcytidine group. The mRNAs simultaneously modified by N1-Methylpseudouridine and 5-Methylcytidine also exhibited reduced activation of the intracellular innate immune response.
Animal experiment:[2]
Animal models	7-week-old Balb/c mice
Dosage form	20 μg Once daily through intradermal or intramuscular routes by lipofection, for 21 days
Applications	N1-Methylpseudouridine alone and its combination with 5-Methylcytidine outperformed the current state-of-the-art Pseudouridine and its combination with 5-Methylcytidine, in terms of translation capacity.
Note	The technical data provided above is for reference only.
	References: 1. Svitkin YV, Cheng YM, Chakraborty T, et al. N1-methyl-pseudouridine in mRNA enhances translation through eIF2α-dependent and independent mechanisms by increasing ribosome density. Nucleic Acids Research, 2017, 45(10): 6023-6036. 2. Andries O, Mc Cafferty S, De Smedt SC, et al N(1)-methylpseudouridine-incorporated mRNA outperforms pseudouridine-incorporated mRNA by providing enhanced protein expression and reduced immunogenicity in mammalian cell lines and mice. Journal of Controlled Release, 2015, 217: 337-344.