Triclosan

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Triclosan

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

3380-34-5

包装与价格：

包装	价格(元)
10mM (in 1mL DMSO)	电议
1g	电议
5g	电议

产品介绍

化学性质

Storage	Store at RT
M.Wt	289.5
Cas No.	3380-34-5
Formula	C₁₂H₇Cl₃O₂
Solubility	insoluble in H2O; ≥10.95 mg/mL in DMSO; ≥47.7 mg/mL in EtOH
Chemical Name	5-chloro-2-(2,4-dichlorophenoxy)-phenol
Canonical SMILES	ClC1=C(OC2=CC=C(Cl)C=C2O)C=CC(Cl)=C1
运输条件	蓝冰运输或根据您的需求运输。
一般建议	为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。

资料参考

Triclosan is a synthetic, lipid soluble broad-spectrum antibacterial and antifungal agent which is widely used in personal care products, household items, medical devices, and fabrics and plastics. It, distributed ubiquitously across the ecosystem, possesses intrinsic oestrogenic and androgenic activity which could provide some explanation for the endocrine disrupting properties described in aquatic species.

In vitro: Triclosan blocked and displaced [3H] oestradiol binding from oestrogen receptors (ER) of MCF7 human breast cancer cells and from recombinant human ERα /ERβ at low concentrations. Triclosan fully dampened the elicitation of the oestrogen-responsive ERE-CAT reporter gene in MCF7 cells and the activation of growth of MCF7 human breast cancer cells by 10-10 M 17β-oestradiol. Additionally, Triclosan, on its own, increased the proliferation of oestrogen-dependent MCF7 human breast cancer cells [1].

In vivo: BALB/c mice were administrated subcutaneously with triclosan daily at 0.8 to 38 mg/kg for 6 weeks. 75% parasitemia was blocked by single subcutaneous injection of triclosan at a dose of 3.0 mg/kg within 24 hours. However, triclosan fully cleared the parasite from circulation with one injection at a dose of 38 mg/kg. No side effects of triclosan were monitored via checking the activities of the enzymes serum glutamate oxaloacetate transaminase and serum glutamate pyruvate transaminase [2].

References:
[1]. Gee, R., Charles, A., Taylor, N., & Darbre, P. Oestrogenic and androgenic activity of triclosan in breast cancer cells. Journal of Applied Toxicology. 2007; 28(1): 78-91.
[2]. Hillyer, C. Triclosan offers protection against blood stages of malaria by inhibiting enoyl-ACP reductase of Plasmodium falciparumM. Surolia, A. Surolia. Nat Med 7:167–173, 2001. Transfusion Medicine Reviews. 2002; 16(2): 180-181.

试验操作

细胞实验 [1]：
细胞系	人类乳腺癌细胞
溶解方法	在DMSO中的溶解度≥10.95mg/mL。为了获得更高的浓度，可以将离心管在37℃加热10分钟和/或在超声波浴中震荡一段时间。原液可以在-20℃以下储存几个月。
反应条件	0～10^-4M
应用	Triclosan阻断并置换来自MCF7人乳腺癌细胞的雌激素受体（ER）和低浓度重组人ERα/ERβ的[3H]雌二醇结合。Triclosan完全抑制了MCF7细胞中雌激素反应性ERE-CAT报告基因的诱导和10-10 M17β-雌二醇激活MCF7人乳腺癌细胞的生长。
动物实验 [2]：
动物模型	BALB / c小鼠
剂量	0.8～38 mg/kg (s.c.) ，每天一次，持续六周
应用	在24小时内单次皮下注射triclosan（剂量为3.0 mg / kg）可阻断75％的寄生虫病。但是，triclosan一次以38 mg / kg的剂量注射完全清除了循环中的寄生虫。通过检查血清谷氨酸草酰乙酸转氨酶和血清谷氨酸丙酮酸转氨酶的活性，未检测到triclosan的副作用。
注意事项	请测试所有化合物在室内的溶解度，实际溶解度和理论值可能略有不同，这是由实验系统的误差引起的，属于正常现象。
References: [1]. Gee RH, Charles A, Taylor N, Darbre PD. Oestrogenic and androgenic activity of triclosan in breast cancer cells. J Appl Toxicol. 2008 Jan;28(1):78-91. doi: 10.1002/jat.1316. PMID: 17992702. [2]. Surolia N, Surolia A. Triclosan offers protection against blood stages of malaria by inhibiting enoyl-ACP reductase of Plasmodium falciparum. Nat Med. 2001 Feb;7(2):167-73. doi: 10.1038/84612. Erratum in: Nat Med 2001 May;7(5)636. PMID: 11175846.