CAS NO: | 142878-12-4 |
包装 | 价格(元) |
5mg | 电议 |
10mg | 电议 |
25mg | 电议 |
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 466.66 |
Cas No. | 142878-12-4 |
Formula | C29H42N2O3 |
Solubility | Soluble in DMSO |
Chemical Name | 1-(6-(((8R,9S,13S,14S,17S)-3-methoxy-13-methyl-7,8,9,11,12,13,14,15,16,17-decahydro-6H-cyclopenta[a]phenanthren-17-yl)amino)hexyl)pyrrolidine-2,5-dione |
Canonical SMILES | C[C@]12CC[C@]3([H])C4=CC=C(OC)C=C4CC[C@@]3([H])[C@]1([H])CC[C@]2([H])NCCCCCCN5C(CCC5=O)=O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
U 73343 is an inactive analog of the putative phospholipase C antagonist U 73122. Phospholipase C is a class of membrane-associated enzymes, which plays an important role in signal transduction pathways by cleaving phosphatidylinositol 4,5-bisphosphate into diacylglycerol and inositol 1,4,5-trisphosphate, which serve as second messengers. Therefore, U 73343 can be used as a negative control of U 73122 for elucidating the role of phospholipase C in a variety of biological events such as gastric acid secretion and hormone-stimulated calcium signals.
References:
1. Muto Y, Nagao T, Urushidani T. The putative phospholipase C inhibitor U73122 and its negative control, U73343, elicit unexpected effects on the rabbit parietal cell. Journal of Pharmacology and Experimental Therapeutics, 1997, 282(3): 1379-1388.
2. Tatrai A, Lee SK, Stern PH. U-73122, a phospholipase C antagonist, inhibits effects of endothelin-1 and parathyroid hormone on signal transduction in UMR-106 osteoblastic cells. Biochimica et Biophysica Acta, 1994, 1224(3): 575-582.
Cell experiment:[2] | |
Cell lines | UMR-106 osteoblastic cells |
Reaction Conditions | 6 ~ 8 μM U 73343 for 3 min incubation |
Applications | At the concentration range of 6 ~ 8 μM, U 73343 elicited no effects on endothelin-1 and parathyroid hormone stimulated intracellular calcium transients, whereas U 73122 exhibited complete inhibition on responses to 100 nM endothelin-1 or parathyroid hormone. Also, U 73343 did not affect endothelin-1-induced inositol trisphosphate production. |
Note | The technical data provided above is for reference only. |
References: 1. Muto Y, Nagao T, Urushidani T. The putative phospholipase C inhibitor U73122 and its negative control, U73343, elicit unexpected effects on the rabbit parietal cell. Journal of Pharmacology and Experimental Therapeutics, 1997, 282(3): 1379-1388. 2. Tatrai A, Lee SK, Stern PH. U-73122, a phospholipase C antagonist, inhibits effects of endothelin-1 and parathyroid hormone on signal transduction in UMR-106 osteoblastic cells. Biochimica et Biophysica Acta, 1994, 1224(3): 575-582. |