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Neomycin sulfate
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Neomycin sulfate图片
CAS NO:1405-10-3
包装与价格:
包装价格(元)
10mM (in 1mL H2O)电议
10g电议

产品介绍

化学性质

Physical AppearanceA solid
StorageStore at -20°C
M.Wt712.72
Cas No.1405-10-3
FormulaC23H46N6O13·H2SO4
Solubility≥33.75 mg/mL in H2O; insoluble in DMSO; insoluble in EtOH
Chemical Name(2R,3R,4R,5R,6R)-5-amino-2-(aminomethyl)-6-[(1R,2S,3S,4R,6S)-4,6-diamino-2-[(2S,3R,4R,5R)-4-[(3R,4R,5R,6S)-3-amino-6-(aminomethyl)-4,5-dihydroxyoxan-2-yl]oxy-3-hydroxy-5-(hydroxymethyl)oxolan-2-yl]oxy-3-hydroxycyclohexyl]oxyoxane-3,4-diol;sulfuric acid
Canonical SMILESC1C(C(C(C(C1N)OC2C(C(C(C(O2)CN)O)O)N)OC3C(C(C(O3)CO)OC4C(C(C(C(O4)CN)O)O)N)O)O)N.OS(=O)(=O)O
运输条件蓝冰运输或根据您的需求运输。
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资料参考

Neomycin sulfate belongs to a kind of aminoglycoside antibiotics, which is a potent inhibitor of hammerhead ribozyme cleavage reaction with aK1 of 13.5 μM.

Neomycin prefers to interact with the ribozyme-substrate complex, which reduces the cleavage rate by stabilizing the ground state of the complex thus destabilizing the transition state of the cleavage process [1]. Neomycin could inhibit the binding of Tat protein to thetrans-activating region (TAR) element of HIV-1 RNA. It turns out that neomycin works as a noncompetitive inhibitor by binding to the Tat-TAR complex and increasing theKoff for dissociation of the peptide from the RNA [2].

Neomycin is the most potent aminoglycoside in stabilizing a DNA triple helix, mainly direct to TAT, and mixed base DNA triplexes, better than known DNA minor groove binders and polyamines. TAT is the preference of triplets stabilized by neomycin, but CGC(+) triplets could be accommodated by it [3]. Neomycin induces a concentration- and voltage-dependent partial block of both the cytosolic and luminal faces of the channels, which shows more appetencies of the luminal area of interaction than the cytosolic area. Dissociation constant (Kb(0)) respectively are 210.20 ± 22.80 and 589.70 ± 184.00 nM for luminal and cytosolic area when zero-voltage. Neomycin also exhibits voltage-dependent relief of block when holding potentials >+ 60 mv [4].

Reference:

[1] Stage T K, Hertel K J, Uhlenbeck O C. Inhibition of the hammerhead ribozyme by neomycin [J]. RNA, 1995, 1(1): 95-101.

[2] Wang S, Huber P W, Cui M, et al. Binding of Neomycin to the TAR Element of HIV-1 RNA Induces Dissociation of Tat Protein by an Allosteric Mechanism [J]. Biochemistry, 1998, 37(16): 5549–5557.

[3] Arya D P, Micovic L, Charles I, et al. Neomycin binding to Watson-Hoogsteen (W-H) DNA triplex groove: a model [J]. Journal of the American Chemical Society, 2003, 125(13): 3733-3744.

[4] Mead F, Williams A J. Block of the ryanodine receptor channel by neomycin is relieved at high holding potentials [J]. Biophysical Journal, 2002, 82(4): 1953-1963.