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Cyanine 3 Tyramide
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Cyanine 3 Tyramide图片
CAS NO:174961-75-2
包装:1mg
市场价:3219元

产品介绍
花青 3 Tyramide (Tyramide-Cy3) 是一种橙色荧光染料,被用作辣根过氧化物酶 (HRP) 催化沉积的报告荧光底物,这是免疫测定和核酸原位杂交中的信号放大技术。
Cas No.174961-75-2
别名氰基-3-酪酰胺,Tyramide-Cy3
Canonical SMILESCCN1C2=C(C=C(C=C2)S(O)(=O)=O)C(/C1=C\C=C\C3=[N+](CCCCCC(NCCC4=CC=C(O)C=C4)=O)C5=C(C=C(C=C5)S([O-])(=O)=O)C3(C)C)(C)C
分子式C39H47N3O8S2
分子量749.94
溶解度Soluble in DMSO
储存条件Store at -20°C
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping ConditionEvaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
产品描述

Orange fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, ISH, FISH and multicolor FISH. HRP catalyzes localized deposition of multiple tyramide molecules (catalyzed reporter deposition, CARD), binding the fluorescein tyramide to adjacent tyrosines to enhance fluorescent signal.

Schmidt et al (1997) Signal amplification in the detection of single-copy DNA and RNA by enzyme-catalyzed deposition (CARD) of the novel fluorescent reporter substrate Cy3.29-tyramide. J.Histochem.Cytochem. 45 365 PMID:9071318 |van Gijlswijk et al (1997) Fluorochrome-labeled tyramides: use in immunocytochemistry and fluorescence in situ hybridization. J.Histochem.Cytochem. 45 375 PMID:9071319 |Hopman et al (1998) Rapid synthesis of biotin-, digoxigenin-, trinitrophenyl-, and fluorochrome-labeled tyramides and their application for in situ hybridization using CARD amplification. J.Histochem.Cytochem. 46 771 PMID:9603790 |Schriml et al (1999) Tyramide signal amplification (TSA)-FISH applied to mapping PCR-labeled probes less than 1 kb in size. Biotechniques 27 608 PMID:10489619 |Zhao et al (2013) Characterization of GABAergic neurons in the mouse lateral septum: a double fluorescence in situ hybridization and immunohistochemical study using tyramide signal amplification. PLoS One 8 e73750 PMID:23967349