Isoprenaline HCl

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Isoprenaline HCl

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

51-30-9

包装与价格：

包装	价格(元)
10mM (in 1mL DMSO)	电议
1g	电议
25g	电议
100g	电议

产品介绍

化学性质

Physical Appearance	A solid
Storage	Store at -20°C
M.Wt	247.72
Cas No.	51-30-9
Formula	C₁₁H₁₇NO₃·HCl
Solubility	≥12.39 mg/mL in DMSO; ≥16.6 mg/mL in EtOH with gentle warming and ultrasonic; ≥50.2 mg/mL in H2O with gentle warming
Chemical Name	4-[1-hydroxy-2-(propan-2-ylamino)ethyl]benzene-1,2-diol;hydrochloride
Canonical SMILES	CC(C)NCC(C1=CC(=C(C=C1)O)O)O.Cl
运输条件	蓝冰运输或根据您的需求运输。
一般建议	为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。

资料参考

Isoprenaline HCl是β-肾上腺素能受体的激动剂[1]。

肾上腺素能受体的作用靶点为去甲肾上腺素和肾上腺素的G蛋白耦联受体。当与肾上腺素反应，β-肾上腺素能受体可引起血管舒张。

在人类脐静脉内皮细胞(HUVECs)内，异丙肾上腺素(100 nM)显著增加连接素Cx43的表达量，也增加了Cx40和Cx37含量。此外，异丙肾上腺素增加耦联细胞的数量。异丙肾上腺素增强分支和复杂分支网络的形成[2]。

在特定隔离的地方刺激鼠输精管，异丙肾上腺素抑制宫缩的EC50值为45.6 nM。在组织中β2-肾上腺素能受体被Timolol最大限度地阻止，异丙肾上腺素抑制收缩的EC50值为1.5 μM[1]。在CFW老鼠，IPR造成最大的骨髓细胞增殖反应，增加了红血球生成活性。这些结果表明异丙肾上腺素激活β-肾上腺素能受体，从而增加了造血干细胞的增殖和分化能力[3]。

参考文献：
[1]. Lotti VJ, Cerino D, Kling P. Characterization of the adrenoreceptor activities of isoprenaline in the field stimulated rat vas deferens: selective supersensitivity to beta 2-mediated responses following reserpine treatment. J Auton Pharmacol, 1982, 2(3): 169-174.
[2]. Dhein S, Gaertner C, Georgieff C, et al. Effects of isoprenaline on endothelial connexins and angiogenesis in a human endothelial cell culture system. Naunyn Schmiedebergs Arch Pharmacol, 2015, 388(1): 101-108.
[3]. Lipski S. Effect of beta-adrenergic stimulation by isoprenaline on proliferation and differentation of mouse bone marrow cells in vivo. Pol J Pharmacol Pharm, 1980, 32(3): 281-287.

试验操作

Cell experiment:[1]
Cell lines	Human umbilical vein endothelial cells (HUVECs)
Reaction Conditions	100 nmol/L isoprenaline for 20 h incubation
Applications	Isoprenaline resulted in significantly enhanced expression of endothelial Cx43 and to a lower degree of Cx40 and Cx37. The number of coupling cells was significantly increased. Regarding angiogenesis, isoprenaline led to significantly enhanced formation of branches and a higher complexity of the tube networks with more branches/length.
Animal experiment:[2]
Animal models	Male albino rats of the Sprague-Dawley strain, 225 ~ 325 g
Dosage form	0.33 mg/kg Administered by subcutaneous injection
Applications	A subcutaneous injection of 0.33 mg/kg isoproterenol decreased blood pressure and increased water intake in nephrectomized rats.
Note	The technical data provided above is for reference only.
	References: 1. Dhein S, Gaertner C, Georgieff C, et al. Effects of isoprenaline on endothelial connexins and angiogenesis in a human endothelial cell culture system. Naunyn-Schmiedeberg's Archives of Pharmacology, 2015, 388(1): 101-108. 2. Hosutt JA, Rowland N, Stricker EM. Hypotension and thirst in rats after isoproterenol treatment. Physiology & Behavior, 1978, 21(4): 593-598.