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α-Amanitin
本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
α-Amanitin图片
CAS NO:23109-05-9
包装:1mg
市场价:2394元

产品介绍

化学性质

Physical AppearanceA solid
StorageStore at -20°C
M.Wt918.97
Cas No.23109-05-9
FormulaC39H54N10O14S
Solubility≥1 mg/mL in H2O; Soluble in Ethanol
Canonical SMILESCCC(C)C1C(=O)NCC(=O)NC2CS(=O)C3=C(CC(C(=O)NCC(=O)N1)NC(=O)C(NC(=O)C4CC(CN4C(=O)C(NC2=O)CC(=O)N)O)C(C)C(CO)O)C5=C(N3)C=C(C=C5)O
运输条件蓝冰运输或根据您的需求运输。
一般建议为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。

资料参考

α-Amanitin是鹅膏蕈中对人类毒性最强的毒素，能够抑制RNA聚合酶II，导致肝肾衰竭。

试验操作

Cell experiment:[1]

Cell lines

Mouse blastocysts

Reaction Conditions

1.1 μg/ml α-amanitin

Applications

α-Amanitin inhibited the incorporation of3H-UMP into embryos. α-Amanitin at the concentration of 1.1 μg/ml was found to inhibit RNA polymerase activity by 32%.

Animal experiment:[1]

Animal models

Preimplantation mouse embryos at the two-cell stage of development collected from CF1 female mice

Dosage form

0 ~ 1 μg/ml α-amanitin

48 h

Applications

Less than 1% of the embryos which had been treated with 1 μg/ml of α-amanitin developed to the morula or blastocyst stages. α-Amanitin can be used to determine which forms of RNA polymerase are active and the relative amounts of each form which are present in preimplantation mouse embryos.

Note

The technical data provided above is for reference only.

References:

1. Warner CM, Versteegh LR. In vivo and in vitro effect of alpha-amanitin on preimplantation mouse embryo RNA polymerase. Nature, 1974, 248(5450): 678-680.