| CAS NO: | 86639-52-3 |
| 包装 | 价格(元) |
| 10mM (in 1mL DMSO) | 电议 |
| 20mg | 电议 |
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 392.4 |
| Cas No. | 86639-52-3 |
| Formula | C22H20N2O5 |
| Solubility | insoluble in H2O; insoluble in EtOH; ≥11.15 mg/mL in DMSO |
| Canonical SMILES | O[C@@]1(CC)C2=C(COC1=O)C(N(CC3=C4N=C(C=C5)C(C=C5O)=C3CC)C4=C2)=O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| Cell experiment:[1] | |
Cell lines | The parental human colon cancer cell line KM12C and its two highly metastatic derivatives KM12SM and KM12L4a |
Reaction Conditions | 2.5 μg/ml 7-ethyl-10-hydroxycamptothecin for 4, 24 or 48 h incubation |
Applications | The exposure of the cells to 7-ethyl-10-hydroxycamptothecin induced S-phase and G2 arrest and the KM12L4a cells had the highest response in a time-dependent manner. The rates of apoptosis in the KM12SM and KM12L4a cell lines were increased time-dependently, though there was no such change in the KM12C cells. In conclusion, the effect of 7-ethyl-10-hydroxycamptothecin on the colon cancer cell lines was mediated via conducting S-phase and G2 arrest and apoptosis. This effect was found in the cell lines with higher metastatic potentials, indicating that 7-ethyl-10-hydroxycamptothecin can be used to treat advanced colon cancers. |
Note | The technical data provided above is for reference only. |
References: 1. Wallin A, Svanvik J, Holmlund B, et al. Anticancer effect of SN-38 on colon cancer cell lines with different metastatic potential. Oncology Reports, 2008, 19(6): 1493-1498. | |
| Description | 7-Ethyl-10-hydroxycamptothecin is an inhibitor of DNA topoisomerase I with IC50 value of 77 nM. | |||||
| Targets | DNA topoisomerase I | |||||
| IC50 | 77 nM | |||||
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