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Cy3 maleimide(non-sulfonated)
本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
Cy3 maleimide(non-sulfonated)图片
包装与价格：
包装价格(元)
5mg电议
25mg电议

产品介绍

化学性质

Storage24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate
M.Wt615.20
FormulaC36H43ClN4O3
Solubility≥16.53 mg/mL in DMSO; ≥3.32 mg/mL in H2O with gentle warming and ultrasonic; ≥8.82 mg/mL in EtOH
Chemical Name1-(6-((2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)amino)-6-oxohexyl)-3,3-dimethyl-2-((1E,3E)-3-(1,3,3-trimethylindolin-2-ylidene)prop-1-en-1-yl)-3H-indol-1-ium
Canonical SMILESCC1(C)C2=CC=CC=C2[N+](CCCCCC(NCCN3C(C=CC3=O)=O)=O)=C1/C=C/C=C4C(C)(C)C5=CC=CC=C5N\4C
运输条件蓝冰运输或根据您的需求运输。
一般建议为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。

资料参考

Cy3 maleimide is a selective and efficient fluorophore dye with low aqueous solubility. This labeling reagent can be used to attach Cy3 fluorophore to proteins and peptides which contain cysteine residues, as well as to other thiolate molecules (such as thiol-containing oligonucleotides). Before the labeling reactions, Cystines should be reduced with TCEP (tris-carboxyethylphosphine). For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer.

MSNs were labeled by Cy3 maleimide named MSN-Cy3s at the concentration of 10 μg ml-1. MSN-Cy3s incubated with MEF-mEGFP cells on a glass coverslip in culture medium for 2 h To further visualize the MSN locations in the cytosol we used super-resolution microscopy (3D SIM). The visually microscope image results confirm that MSNs were internalized by MEF-mEGFP cells [1].

Reference:
[1].  Chiu, H.-Y.; Deng, W.; Engelke, H.; Helma, J.; Leonhardt, H.; Bein, T. Intracellular chromobody delivery by mesoporous silica nanoparticles for antigen targeting and visualization in real time. Scientific Reports, 2016, 6, 25019.

Spectral properties

最大激发光波 (nm)555最大发射光波 (nm)570
消光系数 (M-1cm-1)150000量子产量0.31
CF2600.04CF2800.09