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Pyoluteorin
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Pyoluteorin图片
CAS NO:25683-07-2
包装与价格:
包装价格(元)
1mg电议
5mg电议

产品介绍

化学性质

Physical AppearanceA solid
StorageStore at -20°C
M.Wt272.1
Cas No.25683-07-2
FormulaC11H7Cl2NO3
SynonymsNSC 143092
SolubilitySoluble in DMSO
Chemical Name(4,5-dichloro-1H-pyrrol-2-yl)(2,6-dihydroxyphenyl)-methanone
Canonical SMILESOC1=CC=CC(O)=C1C(C2=CC(Cl)=C(Cl)N2)=O
运输条件蓝冰运输或根据您的需求运输。
一般建议为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。

资料参考

Pyoluteorin is a secondary metabolite produced by the biological control organism Pseudomonas fluorescens Pf-5, a rhizosphere bacterium. Pyoluteorin shows no toxicity to seed- and root-rotting plant pathogens. Pyoluteorin production is influenced by positive autoregulation. Addition of pyoluteorin to liquid cultures of Pf-5 enhanced pyoluteorin production.

Pyoluteorin functions as a signal molecule negatively regulating additional secondary metabolites produced by Pseudomonas [1]. The pyoluteorin contributes to the biological control of soilborne plant diseases by some strains of Pseudomonas fluorescens, including Pf-5. Pyoluteorin induces its own productionand represses the other’s production [2]. Depending on the plant, pyoluteorin production plays an important role in the suppression of damping-off by strain CHA0 without being a major mechanism in disease suppression [3].

References:
[1] Brodhagen M, Henkels M D, Loper J E.  Positive autoregulation and signaling properties of pyoluteorin, an antibiotic produced by the biological control organism Pseudomonas fluorescens Pf-5[J]. Applied and environmental microbiology, 2004, 70(3): 1758-1766.
[2] Kidarsa T A, Goebel N C, Zabriskie T M, et al.  Phloroglucinol mediates cross‐ alk between the pyoluteorin and 2, 4‐diacetylphloroglucinol biosynthetic pathways in Pseudomonas fluorescens Pf‐5[J]. Molecular microbiology, 2011, 81(2): 395-414.
[3] Maurhofer M, Keel C, Haas D, et al.  Pyoluteorin production byPseudomonas fluorescens strain CHA0 is involved in the suppression ofPythium damping-off of cress but not of cucumber[J]. European journal of plant pathology, 1994, 100(3): 221-232.

试验操作

细胞实验 [1]:

细胞系

铜绿假单胞菌

溶解方法

在DMSO中是可溶的。为了获得更高的浓度,可以将离心管在37℃加热10分钟和/或在超声波浴中震荡一段时间。原液可以在-20℃以下储存几个月。

反应条件

0~12500 ng/ml

应用

Pyoluteorin有助于荧光假单胞菌某些菌株(包括Pf-5)对土壤传播的植物病害进行生物防治。Pyoluteorin诱导其自身产生并抑制其他的产生。

References:

[1]. Kidarsa TA, Goebel NC, Zabriskie TM, Loper JE. Phloroglucinol mediates cross-talk between the pyoluteorin and 2,4-diacetylphloroglucinol biosynthetic pathways in Pseudomonas fluorescens Pf-5. Mol Microbiol. 2011 Jul;81(2):395-414. doi: 10.1111/j.1365-2958.2011.07697.x. Epub 2011 May 26. PMID: 21564338.