| 包装: | 10 reactions |
| 市场价: | 2160元 |
The Sulfo-NHS-SS-Biotin Kit contains reagents sufficient for 10 biotin labeling reactions. It is a water-soluble biotin reagent for labeling protein, antibody or other molecules with amidogen (NH2-). The biotin in the kit is sufficient and has been activated for direct use. The whole procedure is simple and quick.
| Sufficient For: 10 labeling reactions, each with 1 to 10 mg of antibody | |||
| Streptavidin | 10 mg | Sulfo-NHS-SS-Biotin | 25 mg |
| HABA Solution | 1 mL | PBS Pack (makes 500 mL) | 1 pack |
| Sephadex G-25 in PD-10 Desalting Columns | 8.3 mL, 10 columns | ||
| Store biotin and streptavidin reagents at -20°C. Store remaining kit components at 4°C. | |||
o Amine-reaction—Reacting with primary amines (-NH2), such as lysine side-chains, or the amino-termini of polypeptides.
o Labeling antibody—This kit can label antibodies to facilitate immobilization, purification or detection.
o Labeling protein—This kit can label proteins to facilitate immobilization, purification or detection.
o Labeling Cell surface molecule—This kit can label the cell surface proteins because the negatively charged reagent does not permeate cell membranes.
o Reversible-Disulfide bond in spacer arm allows the biotin label to be removed using reducing agents such as DTT; only a small sulfhydryl group remains attached to the molecule.
o Medium length—Spacer arm (total length added to target) is 24.3 angstroms; it consists of the native biotin valeric acid group extended by a 7-atom chain
o Solubility increased—Sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds.
Sulfo-NHS-SS-biotin (sulfosuccinimidyl-20(biotinamido)ethyl-1,3-dithiopropionate) is a long-chain cleavable amine-reactive biotinylation reagent. The presence of the negatively charged sulfonate group in the chemical structure of sulfo-NHS-SS-biotin makes it a water-soluble biotinylation reagent that can be directly added to aqueous reactions without prior dissolution of organic solvents. Although no prior dissolution is required, an aqueous stock solution of sulfo-NHS-SS-biotin must be prepared rapidly and used immediately in case of the occurrence of hydrolysis of the active ester. Sulfo-NHS-SS-biotin has been used to react with amine-containing proteins and other molecules forming a complex which further interacts with avidin or streptavidin probes and to purify targeted molecules using affinity chromatography on a column of immobilized avidin or streptavidin.
1）Q：货号K1001-K1006的生物素化试剂盒中，生物素产品与哪些基团反应进行生物素化？
A：该系列试剂盒中生物素试剂（如Sulfo-NHS-LC-Biotin）上的NHS酯与伯胺反应。在蛋白质和多肽中，伯胺基团一般存在于赖氨酸残基的氨基末端和ε胺（侧链）上。
2）Q：带有磺酸基（Sulfo-）的Biotin与不带的Biotin有什么不同？
A：磺酸基（Sulfo-）Biotin可增加水溶性，常用于细胞表面标记；non-sulfonated Biotin需用DMSO或DMF等有机溶剂溶解，可透过细胞膜，常用于标记细胞内带有伯胺（NH2-）的蛋白。
3）Q：K1001-K1006试剂盒中Biotin产品有什么不同？
A：第一，带磺酸基（Sulfo-）的Biotin与不带Sulfo-的Biotin膜渗透性、水溶性不同；第二，(Sulfo) -NHS-Biotin试剂是一种短链生物素化试剂，(Sulfo) -NHS-LC-Biotin试剂是一种中等长度的生物素化试剂，(Sulfo) -NHS-SS-Biotin是一种间隔臂包含可裂解的二硫键的生物素化试剂。
4）HABA分析常见问题
| 常见问题 | 可能原因 | 解决方案 |
| HABA分析时 ΔA500 ≤0 | 蛋白质样品没有生物素化或生物素化水平很低，因为蛋白质上可标记的官能团有限 | 使用替代化学方法（例如巯基反应性-）重复生物素化或使用更高摩尔比的生物素化试剂 |
| 试剂混合不充分 | 稀释前完全溶解并混合HABA/链霉亲和素溶液 | |
| 样品中有颗粒 | 过滤蛋白质样品以去除颗粒 | |
| 生物素化水平偏高 | 未反应的生物素未去除 | 分析前对样品进行透析或脱盐 |
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