| 包装 | 价格(元) |
| 100mg | 电议 |
| 200mg | 电议 |
| 500mg | 电议 |
| 5g | 电议 |
Cell lines | Multiple Myeloma cell line (MM1.S) |
Preparation Method | MM1.S cells were treated with low dose concentrations of cyclophosphamide over a 48 hour time course, after which in vitro cell death was quantified by flow cytometry. |
Reaction Conditions | 2.5, 5, 10, 20μM Cyclophosphamide for 48h. |
Applications | Low-dose cyclophosphamide treatment increased MM cell death in a dose-dependent manner, inducing moderate levels of cell death at 20μM cyclophosphamide after 24 hours and from 10μM cyclophosphamide after 48 hours. |
Animal models | C57BL/6 mice |
Preparation Method | C57BL/6 mice were subcutaneously injected with B16F10 cells and daily treated in a low-dose Cyclophosphamide, Paclitaxel and Docetaxel Animals were monitored every 3 days for tumor growth. |
Dosage form | 10 mg/kg Cyclophosphamide, intraperitoneal(i.p.) injection |
Applications | Cyclophosphamide chemotherapy significantly delays tumor growth as compared to the control group. At day 19, when all animals in both experimental groups were still alive and comparable, mice treated with metronomic chemotherapy showed a 70% reduction in tumor dimension.Such effect was directly correlated with reduction of CD4+ Tcells and parallel increase of CD8+ Tcells |
| 产品描述 | Cyclophosphamide is a frequently used chemotherapy, often in combination with other chemotherapy types, for the treatment of breast cancer, malignant lymphomas, multiple myeloma, and neuroblastoma[1]. Cyclophosphamide’s immunomodulatory function was investigated by conditioning macrophages with tumor cell secretome collected from cyclophosphamide treated MM cell lines. CTX-TCS conditioning augmented the migratory capacity of macrophages and increased CD32 and CD64 Fcγ receptor expression on their cell surface. Daratumumab-specific tumor clearance was increased by conditioning macrophages with CTX-TCS in a dose-dependent manner[2] In vivo，Cyclophosphamide induces early nonapoptotic death of superficial cells, followed by apoptotic death of deeper layers. H&E staining was performed over several days to determine the global urothelial injury and regeneration pattern after cyclophosphamide injection. Compared with uninjured mice, significant sloughing of urothelial cell layers as well as submucosal hemorrhage and inflammation were observed 1 day after cyclophosphamide. cyclophosphamide induces nonapoptotic death of superficial cells starting at 2 hours, followed by apoptotic loss of intermediate and basal cells starting at 4 hours[3] References: |
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