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Biotin-VAD-FMK
本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
Biotin-VAD-FMK图片
CAS NO:1135688-15-1
包装与价格：
包装价格(元)
1mg电议
5mg电议

产品介绍
Biotin-VAD-FMK 是一种细胞可渗透的、不可逆的生物素标记的半胱天冬酶抑制剂，用于鉴定细胞裂解物中的活性半胱天冬酶。
Cas No.1135688-15-1
化学名methyl (3S,6S,9S)-3-(2-fluoroacetyl)-9-isopropyl-6-methyl-5,8,11,18-tetraoxo-22-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)-4,7,10,17-tetraazadocosanoate
Canonical SMILESO=C(N[C@@H](C(C)C)C(N[C@@H](C)C(N[C@H](C(CF)=O)CC(OC)=O)=O)=O)CCCCCNC(CCCC[C@@H]1SC[C@@](N2)([H])[C@]1([H])NC2=O)=O
分子式C30H49FN6O8S
分子量672.8
溶解度DMSO: 20 mM
储存条件Store at -20°C
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping ConditionEvaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
产品描述

Biotin-VAD-FMK is a cell permeable, irreversible biotin-labeled caspase inhibitor, used to identify active caspases in cell lysates.

Biotin-VAD-FMK is a synthetic peptide designed as a methyl ester to facilitate cell permeability.

To examine whether caspase-2 is activated in the absence of proteolytic cleavage, an in vivo affinity labeling approach is used, using the biotinylated caspase inhibitor biotin-VAD-fmk that detects only active caspases[2].

References:
[1]. Li J, et al. Nitric oxide suppresses apoptosis via interrupting caspase activation and mitochondrial dysfunction in cultured hepatocytes. J Biol Chem. 1999 Jun 11;274(24):17325-33.
[2]. Samraj AK, et al. Loss of caspase-9 reveals its essential role for caspase-2 activation and mitochondrial membranedepolarization. Mol Biol Cell. 2007 Jan;18(1):84-93.