资料参考
Cysteine-dependent aspartate-directed proteases (Caspases) are a family of cysteine proteases that play important roles in apoptosis, necrosis, and inflammation. Sequential activation of caspases plays an important role in cell apoptosis. Caspase-6 is a member of the caspase family. Caspase-6 is processed by caspases 7, 8 and 10, and can also undergo self-processing. Caspase-6 acts as a downstream enzyme in the caspase activation cascade and interacts with Caspase 8.
Caspase-6 Fluorometric Assay Kit provides a convenient and simple way for detecting the VEID-dependent caspase activity. VEID-AFC (AFC: 7-amino-4-trifluoromethyl coumarin) emits blue light (λmax = 400 nm); while cleavage of the substrate VEID-AFC by Caspase-6 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified by using a fluorecence microtiter plate reader or a fluorometer. Comparison of the fluorescence of free AFC from an apoptotic sample with an uninduced control determines the fold increase in Caspase-6 activity.
Features & Properties
| Features | Fast and convenient
Simple one-step procedure; takes 1-2 hours.
Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-6 activity. |
| Shipping | Gel pack |
| Storage Conditions | Store at -20°C. |
概述
| Species reactivity | Mammalian |
| Applications | Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis. |
| Kit components | Cell Lysis Buffer
2X Reaction Buffer
VEID-AFC (1 mM)
DTT (1 M) |
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