| 包装 | 价格(元) |
| 20assays | 电议 |
| 50assays | 电议 |
| 100assays | 电议 |
| Storage | 2-8°C保存，Annexin V-FITC和Propidium Iodide需要避光保存 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
Annexin V是一种Ca2+依赖性磷脂结合蛋白，可与磷脂酰丝氨酸（Phosphatidylserine, PS）特异结合。PS正常位于细胞膜脂质双层的内侧，但在细胞凋亡早期PS可从细胞膜的内侧翻转到细胞膜的外表面，暴露在细胞外环境中。Annexin V对PS具有高亲和力，对Annexin V进行荧光素（FITC）标记，以带有标记的Annexin V作为荧光探针，利用流式细胞仪或荧光显微镜可检测细胞凋亡的发生，染色过程仅需10-20分钟。碘化丙啶（Propidium Iodide, PI）可与双链DNA特异性结合产生红色荧光，正常情况下无法透过细胞膜。但在凋亡晚期的细胞和死细胞，PI能够透过细胞膜而使细胞核染色。当同时进行Annexin V-FITC和PI染色时可区分处于不同凋亡时期的细胞及死细胞。
| Components | K2003-20 20 Assays | K2003-50 50 Assays | K2003-100 100 Assays |
| Annexin V-FITC | 100 μL | 250 μL | 500 μL |
| 1X Binding Buffer | 10 mL | 25 mL | 50 mL |
| Propidium Iodide | 100 μL | 250 μL | 500 μL |
2-8°C保存，Annexin V-FITC和Propidium Iodide需要避光保存，有效期半年。Annexin V-FITC不可冷冻保存。 | |||
Annexin V is a cellular protein, which plays important roles in the inhibition of the activity of phospholipase A1 and blood coagulation by competing for phosphatidylserine (PS) binding sites with prothrombin. Annexin V has a high affinity to PS and is used as a probe to detect cells that have expressed PS on the cell surface. After initiating apoptosis, cells translocate phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface soon, which can be easily detected by Annexin V.
Annexin V-FITC Apoptosis Detection Kit uses a fluorescent conjugate of Annexin V that can easily detect PS on the cell surface after initiating apoptosis. The one-step staining procedure needs only 10 minutes. The result can be analyzed by fluorescence microscopy or by flow cytometry. The Annexin V-FITC Apoptosis Detection Kit can differentiate apoptosis vs necrosis when performing both Annexin V-FITC and PI staining.
快速：在10-20分钟内完成染色。
方便：适用于流式细胞仪和荧光显微镜检测。
准确：当进行Annexin V-FITC和PI双染色时，Kit可以准确区分活细胞、凋亡细胞和坏死细胞。
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