| CAS NO: | 24939-03-5 |
| 包装 | 价格(元) |
| 10mM (in 1mL H2O) | 电议 |
| 10mg | 电议 |
| 50mg | 电议 |
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| Cas No. | 24939-03-5 |
| Formula | (C10H13N4O8P)x.(C9H14N3O8P)x |
| Solubility | insoluble in DMSO; insoluble in EtOH; ≥21.5 mg/mL in H2O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
Poly (I:C), a synthetic double-stranded RNA (dsRNA) analog, is an immunostimulant that acts as the most potent interferon (IFN) inducer. Toll-like receptor- 3 (TLR3) agonist[1].
Mixtures that included the TLR7/8 agonists R848 or CL075, combined with the Poly (I:C), yield 3-d mature dendritic cells [2].
Commonly used to stimulate immune system activationin vivo. Also promotes maturation of hPSC-derived cardiomyocytes[3].
References:
[1]. Li Y G, Siripanyaphinyo U, Tumkosit U, et al. Poly (I:C), an agonist of toll-like receptor-3, inhibits replication of the Chikungunya virus in BEAS-2B cells. Virology Journal, 2012, 9(1): 114.
[2]. Spranger S, Javorovic M, Burdek M, et al. Generation of Th1-Polarizing Dendritic Cells Using the TLR7/8 Agonist CL075. The Journal of Immunology, 2010, 185(1): 738-747.
[3].Biermann M1,Cai W2,Lang D, et al. Epigenetic Priming of Human Pluripotent Stem Cell-Derived Cardiac Progenitor Cells Accelerates Cardiomyocyte Maturation. Stem Cells, 2019, 37(7): 910-923.
| 细胞实验 [1]: | |
细胞系 | 树突状细胞 |
溶解方法 | 可溶于水。若获取更高浓度的溶液，可在37℃下孵育10分钟，随后在超声波浴中摇匀。-20℃以下可储存数月。 |
反应条件 | 12.5 mg/ml, 3天 |
应用 | 用poly（I：C）处理未成熟DC 72小时诱导产生典型的DC形态改变并显著诱导DC成熟。清除细胞因子后，poly(I:C)处理的DC表型稳定。Poly（I：C）处理的DC胞吞活性下调。 Poly（I：C）处理的DC产生高水平的IL-12和低水平的IL-10。 |
注意事项 | 由于实验环境的不同，实际溶解度可能与理论值略有不同，请测试室内所有化合物的溶解度。 |
References: [1]. Verdijk R M, Mutis T, Esendam B, et al. Polyriboinosinic polyribocytidylic acid (poly (I: C)) induces stable maturation of functionally active human dendritic cells[J]. The Journal of Immunology, 1999, 163(1): 57-61. | |
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