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Chloroquine phosphate
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Chloroquine phosphate图片
CAS NO:50-63-5
包装与价格:
包装价格(元)
100mg电议
200mg电议
500mg电议
1 g电议
5 g电议

产品名称
磷酸氯喹
产品介绍
Chloroquine phosphate 是一种广泛用于疟疾和类风湿性关节炎的抗疟疾和抗炎剂。Chloroquine phosphate 是autophagytoll-like receptors (TLRs)的抑制剂。Chloroquine phosphate 有效抑制SARS-CoV-2(COVID-19) 感染 (EC50=1.13 μM)。
生物活性

Chloroquine phosphate is anantimalarialand anti-inflammatory agent widely used to treat malaria and rheumatoid arthritis. Chloroquine phosphate is anautophagyandtoll-like receptors (TLRs)inhibitor. Chloroquine phosphate is highly effective in the control ofSARS-CoV-2 (COVID-19)infectionin vitro (EC50=1.13 μM)[1][2][3][4].

IC50& Target[1][2][3][5]

Plasmodium

 

Malaria

 

TLRs

 

SARS-COV-2

 

HIV-1

 

体外研究
(In Vitro)

Chloroquine (CHQ, 20 μM) inhibits IL-12p70 release and reduces Th1-priming capacity of activated human monocyte-derived Langerhans-like cells (MoLC). Chloroquine (CHQ, 20 μM) enhances IL-1–induced IL-23 secretion in MoLC and subsequently increases IL-17A release by primed CD4+T cells[1]. Chloroquine (25 μM) suppresses MMP-9 mRNA expression in normoxia and hypoxia in parental MDA-MB-231 cells. Chloroquine has cell-, dose- and hypoxia-dependent effects on MMP-2, MMP-9 and MMP-13 mRNA expression[2]. TLR7 and TLR9 inhibition using IRS-954 or chloroquine significantly reduces HuH7 cell proliferation in vitro[3].
Chloroquine (0.01-100 μM; 48 hours) potently blocks virus infection (vero E6 cells infected with SARS-CoV-2) at low-micromolar concentration (EC50= 1.13 μM). Chloroquine blocks virus infection by increasing endosomal pH required for virus/cell fusion, as well as interfering with the glycosylation of cellular receptors of SARS-CoV[4].

体内研究
(In Vivo)

Chloroquine (80 mg/kg, i.p.) does not prevent the growth of the triple-negative MDA-MB-231 cells with high or low TLR9 expression levels in the orthotopic mouse model[2]. TLR7 and TLR9 inhibition using IRS-954 or chloroquine significantly inhibits tumour growth in the mouse xenograft model. HCC development in the DEN/NMOR rat model is also significantly inhibited by chloroquine[3].

Clinical Trial
分子量

515.86

性状

Solid

Formula

C18H32ClN3O8P2

CAS 号

50-63-5

中文名称

磷酸氯喹;氯喹宁二磷酸盐;氯化喹啉磷酸盐;氯喹磷酸盐;磷酸氯化喹啉;磷酸氯喹啉;二磷酸氯喹;氯喹二磷酸盐

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性数据
In Vitro: 

H2O : ≥ 33 mg/mL(63.97 mM)

DMSO :< 1 mg/mL (ultrasonic)(insoluble or slightly soluble)

*"≥" means soluble, but saturation unknown.

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM1.9385 mL9.6926 mL19.3851 mL
5 mM0.3877 mL1.9385 mL3.8770 mL
10 mM0.1939 mL0.9693 mL1.9385 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture and light)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: PBS

    Solubility: 100 mg/mL (193.85 mM); Clear solution; Need ultrasonic and warming and heat to 60℃

*以上所有助溶剂都可在本网站选购。