Acetylcysteine (N-Acetylcysteine) is amucolytic agentwhich reduces the thickness of the mucus. Acetylcysteine is aROSinhibitor^[1]. Acetylcysteine is a cysteine precursor, prevents hemin-inducedferroptosisby neutralizing toxic lipids generated by arachidonate-dependent activity of 5-lipoxygenases^[5]. Acetylcysteine induces cellapoptosis^[2][3]. Acetylcysteine also has anti-influenza virusactivities^[7].

Acetylcysteine prevents apoptotic DNA fragmentation and maintains long-term survival in the absence of other trophic support in serum-deprived PC12 cells. Acetylcysteine also prevents death of PC12 cells and sympathetic neurons^[2].
Acetylcysteine causes dose-dependent reductions in viability in rat and human aortic smooth muscle cells^[3].
Acetylcysteine activates the Ras-extracellular signal-regulated kinase (ERK) pathway in PC12 cells. Acetylcysteine protects neuronal cells from death evoked by withdrawal of trophic support. Acetylcysteine increases nitric oxide (NO) release from protein-bound stores in vascular tissue. Acetylcysteine pretreatment of PC12 cells interferes with NGF-dependent signaling and neurite outgrowth, and it is suggested that Acetylcysteine interferes with redox-sensitive steps in the NGF mechanism^[4].

Acetylcysteine (150, 300 mg/kg) treatment significantly reduces liver transaminases in all groups of treatment, mostly in group Acetylcysteine 300. Lung glutathione peroxidase is significantly increases in group Acetylcysteine 300 (P=0.04), while the other oxidation biomarkers show no significant differences^[6].
Acetylcysteine improves cognition of 12-month-old SAMP8 mice in both the T-maze footshock avoidance paradigm and the lever press appetitive task without inducing non-specific effects on motor activity, motivation to avoid shock, or body weight^[5].

163.19

Solid

C₅H₉NO₃S

616-91-1

乙酰半胱氨酸

• Ketones, Aldehydes, Acids

Room temperature in continental US; may vary elsewhere.

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

H₂O : 163 mg/mL(998.84 mM;ultrasonic and adjust pH to 7 with NaOH)

DMSO : ≥ 100 mg/mL(612.78 mM)

*"≥" means soluble, but saturation unknown.

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (protect from light)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂： PBS

  Solubility: 120 mg/mL (735.34 mM); Clear solution; Need ultrasonic