Mollugin

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Mollugin

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

55481-88-4

包装与价格：

包装	价格(元)
5mg	电议
10mg	电议
20mg	电议
50mg	电议
100mg	电议

产品名称

大叶茜草素

产品介绍

Mollugin 是一种口服有效的NF-κB抑制剂。Mollugin 诱导 HepG2 细胞 S 期阻滞，增加细胞内活性氧 (ROS) 水平。Mollugin 诱导 HepG2 细胞 DNA 损伤，并增加 p-H2AX 的表达。Mollugin 通过抑制 TNF-α 诱导的 NF-κB 活化而具有抗癌作用。Mollugin 通过 p38-Smad 信号通路增强 BMP-2 (骨形态发生蛋白 2) 的成骨作用。

生物活性

Mollugin is an orally active and potentNF-κBinhibitor. Mollugin induces S-phase arrest of HepG2 cells, and increased intracellularreactive oxygen species(ROS) levels. Mollugin induces DNA damage in HepG2 cells, as well as an increase in the expression of p-H2AX. Mollugin shows anti-cancer effect by inhibiting TNF-α-inducedNF-κBactivation. Mollugin enhances the osteogenic action of BMP-2 (bone morphogenetic protein 2) via the p38-Smad signaling pathway^[1]^[2]^[3].

体外研究
(In Vitro)

Mollugin (0-80 μM, 24 h) inhibits the expression of an NF-κB reporter gene induced by TNF-α in a dose-dependent manner^[1].
Mollugin (0-80 μM, 12 h) inhibits the proliferation of HeLa cells^[1].
Mollugin (0-80 μM, 12 h) inhibits TNF-α-induced phosphorylation and nuclear translocation of p65, phosphorylation and degradation of IκBα, and IKK phosphorylation, and inhibits the TNF-α-induced mRNA expression of Cyclin D1, c-Myc, and VEGF^[1].
Mollugin (0-80 μM, 12 h) enhances TNF-α-induced apoptosis^[1].

Cell Viability Assay^[1]

Cell Line:	HeLa, Hep3B, and HEK293 cells
Concentration:	0, 10, 20, 40, and 80 μM
Incubation Time:	24 h
Result:	Significantly inhibited NF-κB reporter gene expression in a dose-dependent manner, did not display significant cellular toxicity in HeLa, Hep3B, and HEK293 cells.

Cell Proliferation Assay^[1]

Cell Line:	HeLa cells
Concentration:	0, 20, 40, and 80 μM
Incubation Time:	12 h
Result:	Inhibited the proliferation of HeLa cells.

Apoptosis Analysis^[1]

Cell Line:	HeLa cells
Concentration:	0, 10, 20, 40, and 80 μM
Incubation Time:	12 h
Result:	Slightly affected the caspase-3 activation, potentiated the effect of TNF-α-induced PARP cleavage, and enhanced the apoptotic effects of TNF-α.

Western Blot Analysis^[1]

Cell Line:	HeLa cells
Concentration:	0, 10, 20, 40, and 80 μM
Incubation Time:	12 h
Result:	Significantly inhibited the TNF-α-induced p65 phosphorylation and block TNF-α-induced nuclear translocation of p65 in a dose-dependent manner, completely inhibited degradation of IκBα at 80 μM, and abolished the TNF-α-induced IKK phosphorylation at 80 μM. Inhibited the TNF-α-induced mRNA expression of Cyclin D1, c-Myc, and VEGF at 80 μM.

体内研究
(In Vivo)

Mollugin (0-75 mg/kg, Orally, three times per week for 36 days) inhibits growth of HeLa cells in a xenograft tumor model^[1].

Animal Model:	BALB/c female athymic nude mice (six-week-old, subcutaneously injected with 0.2 mL HeLa cells)^[1]
Dosage:	0, 25 and 75 mg/kg
Administration:	Orally, three times per week for 36 days
Result:	Suppressed tumor growth, whereas the body weight did not change. Significantly reduced the protein expression of p-p65 and COX-2 in the tumors.

分子量

284.31

性状

Solid

Formula

C₁₇H₁₆O₄

CAS 号

55481-88-4

中文名称

大叶茜草素

结构分类

Terpenoids
Sesquiterpenes

Phenols
Monophenols

来源

Plants
Rubiaceae
Rubia cordifoliaL.

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据

In Vitro:

DMSO : 33.33 mg/mL(117.23 mM;Need ultrasonic)

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	3.5173 mL	17.5864 mL	35.1729 mL
5 mM	0.7035 mL	3.5173 mL	7.0346 mL
10 mM	0.3517 mL	1.7586 mL	3.5173 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (protect from light)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.
请依序添加每种溶剂： 10% DMSO 40%PEG300 5%Tween-80 45% saline
Solubility: ≥ 2.5 mg/mL (8.79 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (8.79 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH2O 中，得到澄清透明的生理盐水溶液
2.
请依序添加每种溶剂： 10% DMSO 90%corn oil
Solubility: ≥ 2.5 mg/mL (8.79 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (8.79 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在本网站选购。