Oxidopamine (6-OHDA) hydrochloride 是神经递质多巴胺 (neurotransmitter dopamine) 的拮抗剂。Oxidopamine hydrochloride 是一种广泛应用的神经毒素，可选择性破坏多巴胺能神经元。Oxidopamine hydrochloride 促进COX-2激活，导致PGE2合成和促炎细胞因子IL-1β的分泌。Oxidopamine hydrochloride 可用于帕金森病 (PD)、注意缺陷多动障碍 (ADHD) 和莱施奈恩综合症的研究。
| 生物活性 | Oxidopamine (6-OHDA) hydrochloride is an antagonist of theneurotransmitter dopamine. Oxidopamine hydrochloride is a widely used neurotoxin and selectively destroys dopaminergic neurons. Oxidopamine hydrochloride promotesCOX-2activation, leading toPGE2synthesis and pro-inflammatory cytokineIL-1βsecretion. Oxidopamine hydrochloride can be used for the research of Parkinson’s disease (PD), attention-deficit hyperactivity disorder (ADHD), and Lesch-Nyhan syndrome[1][2][3][4]. |
| IC50& Target | COX-2 | IL-1β | Caspase-3 | Caspase-8 | Caspase-9 |
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体外研究
(In Vitro) | Oxidopamine hydrochloride (0-500 μM, 24 h) decreases the viability of both Neuro-2a cells and SH-SY5Y cells in a concentration-dependent manner[1].
Oxidopamine hydrochloride (75-150 μM, 0-24 h) induces COX-2 expression and nuclear translocation[1].
Oxidopamine hydrochloride (75-150 μM, 0-24 h) causes PGE2biosynthesis and pro-inflammatory cytokine IL-1β production[1].
Oxidopamine hydrochloride (0-150 μM, 12 h) inducesapoptosisand mitochondrial membrane depolarization of pheochromocytoma PC12 cells[3].
Oxidopamine hydrochloride (75 μM, 0-12 h) induces p38 phosphorylation[3].
Cell Viability Assay[1] | Cell Line: | Neuro-2a cells and SH-SY5Y cells | | Concentration: | 0-500 μM | | Incubation Time: | 24 or 48 h | | Result: | Induced neurotoxicity, caused cytotoxicity in both Neuro-2a cells and SH-SY5Y cells in a concentration dependent manner. EC50=111 μM for 24 h incubation and 109 μM for 48 h incubation in the Neuro-2a cells; EC50=118 μM for 24 h incubation and 107 μM for 48 h incubation in the SH-SY5Y cells. |
RT-PCR[1] | Cell Line: | Neuro-2a cells and SH-SY5Y cells | | Concentration: | 75 or 150 μM | | Incubation Time: | 0, 6 or 24 h | | Result: | Quickly and robustly induced COX-2 in a time-dependent manner. Induced COX-2 activation characterized by expression induction and nuclear translocation. Substantially increased PGE2in the culture medium by nearly 5-fold in Neuro-2a cells (at 75 μM) and 3-fold in SH-SY5Y cells (at 150 μM). Significantly upregulated the pro-inflammatory cytokine interleukin-1β (IL-1β) within Neuro-2a cells and SH-SY5Y cells. |
Apoptosis Analysis[3] | Cell Line: | PC12 cells | | Concentration: | 0, 25, 50, 75, and 150 μM | | Incubation Time: | 0, 2, 4, 6, 12, and 20 h | | Result: | Induced apoptosis of PC12 cells. Increased the activities of caspase-3, -8 and -9 in PC12 cells in a time- and concentration-dependent manner. Increased these caspase activities at 2-4 h and reached a maximum at 12 h. Decreased cells with high mitochondrial membrane potential (JC-1 aggregate) in a time- and concentration-dependent manner. |
Western Blot Analysis[3] | Cell Line: | PC12 cells | | Concentration: | 75 μM | | Incubation Time: | 0, 3, 5, 6, 8, 10, and 12 h | | Result: | Increased the level of p-p38 in a time-dependent manner. |
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体内研究
(In Vivo) | Oxidopamine hydrochloride (5 μg/2 μL, unilaterally injected into the right striatum) induces degeneration of dopaminergic neurons in substantia nigra of rats[2].
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | 4°C, stored under nitrogen *该产品在溶液状态不稳定，建议您现用现配，即刻使用。 |
| 溶解性数据 | In Vitro: H2O : 100 mg/mL(486.29 mM;Need ultrasonic) DMSO : 83.33 mg/mL(405.22 mM;Need ultrasonic) 配制储备液 | 1 mM | 4.8629 mL | 24.3143 mL | 48.6287 mL | | 5 mM | 0.9726 mL | 4.8629 mL | 9.7257 mL | | 10 mM | 0.4863 mL | 2.4314 mL | 4.8629 mL |
*请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液；该产品在溶液状态不稳定，建议您现用现配，即刻使用。 In Vivo: 请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂： ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶 1. 请依序添加每种溶剂： PBS Solubility: 100 mg/mL (486.29 mM); Clear solution; Need ultrasonic 2. 请依序添加每种溶剂： 10% DMSO 40%PEG300 5%Tween-80 45% saline Solubility: ≥ 2.08 mg/mL (10.11 mM); Clear solution
此方案可获得 ≥ 2.08 mg/mL (10.11 mM，饱和度未知) 的澄清溶液。 以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。 3. 请依序添加每种溶剂： 10% DMSO 90% (20%SBE-β-CDin saline) Solubility: ≥ 2.08 mg/mL (10.11 mM); Clear solution
此方案可获得 ≥ 2.08 mg/mL (10.11 mM，饱和度未知) 的澄清溶液。 以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。 *以上所有助溶剂都可在本网站选购。
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