Amifostine

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Amifostine

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

20537-88-6

包装与价格：

包装	价格(元)
10 mM * 1 mL in Water	电议
10mg	电议
50mg	电议
100mg	电议
200mg	电议
500mg	电议
1 g	电议

产品名称

氨磷汀
WR2721

产品介绍

Amifostine (WR2721) 是一种广谱细胞保护剂和辐射防护剂。Amifostine 可选择性保护正常组织免受放射线和化学疗法造成的损害。Amifostine 是有效的HIF-α1和p53诱导剂。Amifostine 通过清除氧衍生的自由基来保护细胞免受损伤。Amifostine 可降低肾脏毒性并具有抗血管生成作用。

生物活性

Amifostine (WR2721) is a broad-spectrum cytoprotective agent and a radioprotector. Amifostine selectively protects normal tissues from damage caused by radiation and chemotherapy. Amifostine is potenthypoxia-inducible factor-α1 (HIF-α1)andp53inducer. Amifostine protects cells from damage by scavenging oxygen-derived free radicals. Amifostine reduces renal toxicity and has antiangiogenic action^[1]^[2]^[3]^[4].

体外研究
(In Vitro)

Amifostine (0.78125-100 μM, 24 h) reduces tert-Butyl hydroperoxide (TBHP)-induced cell damage in a dose-dependent manner and significantly reduces H9c2 cells apoptosis at a concentration of 100 μM^[5].

体内研究
(In Vivo)

Amifostine (i.v., 400 mg/kg, 4 h) has a protective effect against myocardial I/R injury in male C57BL/6 mice^[5].

Animal Model:	Male C57BL/6 mice with myocardial I/R injury^[5]
Dosage:	400 mg/kg
Administration:	Intravenous injection; 4 hours
Result:	Attenuated cardiomyocyte apoptosis and reduced the production of I/R-induced ROS. Significantly reduced the expression of cleaved caspase 3 and Bax while enhanced the expression of SOD1, SOD2 and Bcl2. Significantly increased SOD activity and reduced MDA levels.

Clinical Trial

分子量

214.22

性状

Solid

Formula

C₅H₁₅N₂O₃PS

CAS 号

20537-88-6

中文名称

氨磷汀；安磷汀；阿米福汀

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

In Vitro:

H₂O : 100 mg/mL(466.81 mM;Need ultrasonic)

DMF : 1 mg/mL(4.67 mM;Need ultrasonic)

DMSO :< 1 mg/mL (ultrasonic)(insoluble or slightly soluble)

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	4.6681 mL	23.3405 mL	46.6810 mL
5 mM	0.9336 mL	4.6681 mL	9.3362 mL
10 mM	0.4668 mL	2.3340 mL	4.6681 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.
请依序添加每种溶剂： PBS
Solubility: 100 mg/mL (466.81 mM); Clear solution; Need ultrasonic and warming and heat to 60℃

*以上所有助溶剂都可在本网站选购。